Please use this identifier to cite or link to this item: https://doi.org/10.1186/1471-2334-13-165
Title: Multiple-antigen ELISA for melioidosis - a novel approach to the improved serodiagnosis of melioidosis
Authors: Hara, Y.
Chin, C.-Y.
Mohamed, R.
Puthucheary, S.D. 
Nathan, S.
Keywords: ELISA
Melioidosis
Serodiagnosis
TssD-5 and Omp3
Issue Date: 4-Apr-2013
Citation: Hara, Y., Chin, C.-Y., Mohamed, R., Puthucheary, S.D., Nathan, S. (2013-04-04). Multiple-antigen ELISA for melioidosis - a novel approach to the improved serodiagnosis of melioidosis. BMC Infectious Diseases 13 (1) : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2334-13-165
Abstract: Background: Burkholderia pseudomallei, the causative agent of melioidosis, is endemic to Southeast Asia and northern Australia. Clinical manifestations of disease are diverse, ranging from chronic infection to acute septicaemia. The current gold standard of diagnosis involves bacterial culture and identification which is time consuming and often too late for early medical intervention. Hence, rapid diagnosis of melioidosis is crucial for the successful management of melioidosis.Methods: The study evaluated 4 purified B. pseudomallei recombinant proteins (TssD-5, Omp3, smBpF4 and Omp85) as potential diagnostic agents for melioidosis. A total of 68 sera samples from Malaysian melioidosis patients were screened for the presence of specific antibodies towards these proteins using enzyme-linked immunosorbent assay (ELISA). Sera from patients with various bacterial and viral infections but negative for B. pseudomallei, as well as sera from healthy individuals, were also included as non-melioidosis controls. The Mann Whitney test was performed to compare the statistical differences between melioidosis patients and the non-melioidosis controls.Results: TssD-5 demonstrated the highest sensitivity of 71% followed by Omp3 (59%), smBpF4 (41%) and Omp85 (19%). All 4 antigens showed equally high specificity (89-96%). A cocktail of all 4 antigens resulted in slightly reduced sensitivity of 65% but improved specificity (99%). Multiple-antigen ELISA provided improved sensitivity of 88.2% whilst retaining good specificity (96%). There was minimal reactivity with sera from healthy individuals proposing the utility of these antigens to demarcate diseased from non-symptomatic individuals in an endemic country.Conclusions: TssD-5 demonstrated high detection sensitivity and specificity and the results were obtained within a few hours compared to time consuming culture and IFAT methods commonly used in a clinical setting. The use of multiple-antigens resulted in improved sensitivity (88.2%) whilst maintaining superior specificity. These data highlight the use of TssD-5 and other recombinant antigens tested in this study as potential serodiagnostic agents for melioidosis. © 2013 Hara et al.; licensee BioMed Central Ltd.
Source Title: BMC Infectious Diseases
URI: http://scholarbank.nus.edu.sg/handle/10635/126520
ISSN: 14712334
DOI: 10.1186/1471-2334-13-165
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

20
checked on Dec 2, 2022

WEB OF SCIENCETM
Citations

17
checked on Dec 2, 2022

Page view(s)

287
checked on Dec 1, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.