Please use this identifier to cite or link to this item: https://doi.org/10.7883/yoken.67.157
Title: Molecular analysis of ciprofloxacin resistance among non-typhoidal Salmonella with reduced susceptibility to ciprofloxacin isolated from patients at a tertiary care Hospital in Kuala Lumpur, Malaysia
Authors: Karunakaran, R.
Tay, S.T.
Rahim, F.F.
Lim, B.B.
Puthucheary, S.D. 
Keywords: Ciprofloxacin
Malaysia
Molecular analysis
Non-Typhoidal Salmonella
Resistance
Issue Date: 2014
Citation: Karunakaran, R., Tay, S.T., Rahim, F.F., Lim, B.B., Puthucheary, S.D. (2014). Molecular analysis of ciprofloxacin resistance among non-typhoidal Salmonella with reduced susceptibility to ciprofloxacin isolated from patients at a tertiary care Hospital in Kuala Lumpur, Malaysia. Japanese Journal of Infectious Diseases 67 (3) : 157-162. ScholarBank@NUS Repository. https://doi.org/10.7883/yoken.67.157
Abstract: We investigated the prevalence of non-typhoidal Salmonella (NTS) with "reduced susceptibility to ciprofloxacin" (RS-Cip) (minimum inhibitory concentration [MIC], 0.12-1.0 μg/mL) as well as their resistance genes in 75 NTS isolates (53 from stool, 21 from blood, and 1 from urine) from patients at a tertiary care Malaysian hospital between January and December 2009. RS-Cip was detected in 24/75 (32.0z) isolates. Using the ciprofloxacin MIC interpretive criteria for Salmonella in the Clinical and Laboratory Standards Institute 2013 guidelines, 51/75 (68.0z) isolates were found to be sensitive, 22/75 (29.3z) were intermediate, and 2/75 (2.7z) were resistant to ciprofloxacin. The 24 isolates that were intermediate or resistant to ciprofloxacin were the same isolates categorized as having RS-Cip. Among the 23 tested isolates with RS-Cip, the qnrS gene was detected in 17/23 (73.9z) and single gyrA mutations were detected in 6/23 (26.1z) (Asp87Tyr [n = 3], Asp87Asn [n = 2], and Ser83Phe [n = 1]). A parC (Thr57Ser) mutation was detected in 13/23 (56.5z) isolates, coexisting with either a qnrS gene or a gyrA mutation. The high incidence of the qnrS gene among isolates with RS-Cip needs to be monitored because qnr genes can spread via plasmids and aid in the emergence of increased resistance levels.
Source Title: Japanese Journal of Infectious Diseases
URI: http://scholarbank.nus.edu.sg/handle/10635/126518
ISSN: 18842836
DOI: 10.7883/yoken.67.157
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

6
checked on Dec 7, 2022

WEB OF SCIENCETM
Citations

7
checked on Dec 7, 2022

Page view(s)

164
checked on Dec 1, 2022

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.