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|Title:||Mad2 inhibits the mitotic kinesin MKlp2||Authors:||Lee, S.H.
|Issue Date:||13-Dec-2010||Citation:||Lee, S.H., McCormick, F., Saya, H. (2010-12-13). Mad2 inhibits the mitotic kinesin MKlp2. Journal of Cell Biology 191 (6) : 1069-1077. ScholarBank@NUS Repository. https://doi.org/10.1083/jcb.201003095||Abstract:||We identified the mitotic kinesin-like protein 2 (MKlp2), a kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis, as a target of the mitotic checkpoint protein Mad2. MKlp2 possesses a consensus Mad2-binding motif required for Mad2 binding. Mad2 prevents MKlp2 from loading onto the mitotic spindle, a prerequisite step for its function as a mitotic kinesin. Furthermore, Mad2 inhibits the ability of MKlp2 to relocate the CPC from centromeres, an essential step to promote cytokinesis. An MKlp2 mutant that is refractory to Mad2-mediated inhibition prematurely translocates to the mitotic spindle and mislocalizes the CPC component Aurora B from the midbody of dividing cells. This correlates with an increased incidence of cytokinesis failure. Together, these findings reveal that MKlp2 is a novel mitotic target of Mad2 necessary for proper mitotic progression and cytokinesis. © 2010 Lee et al.||Source Title:||Journal of Cell Biology||URI:||http://scholarbank.nus.edu.sg/handle/10635/126512||ISSN:||00219525||DOI:||10.1083/jcb.201003095|
|Appears in Collections:||Staff Publications|
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