Please use this identifier to cite or link to this item:
Title: Large-scale ex vivo expansion and characterization of natural killer cells for clinical applications
Authors: Lapteva, N.
Durett, A.G.
Sun, J.
Rollins, L.A.
Huye, L.L.
Fang, J.
Dandekar, V.
Mei, Z.
Jackson, K.
Vera, J.
Ando, J.
Ngo, M.C.
Coustan-Smith, E. 
Campana, D. 
Szmania, S.
Garg, T.
Moreno-Bost, A.
Vanrhee, F.
Gee, A.P.
Rooney, C.M.
Keywords: Cell-based
Ex vivo expansion
Gas-permeable static cell culture flasks (G-Rex)
Natural killer cells
Issue Date: Oct-2012
Citation: Lapteva, N., Durett, A.G., Sun, J., Rollins, L.A., Huye, L.L., Fang, J., Dandekar, V., Mei, Z., Jackson, K., Vera, J., Ando, J., Ngo, M.C., Coustan-Smith, E., Campana, D., Szmania, S., Garg, T., Moreno-Bost, A., Vanrhee, F., Gee, A.P., Rooney, C.M. (2012-10). Large-scale ex vivo expansion and characterization of natural killer cells for clinical applications. Cytotherapy 14 (9) : 1131-1143. ScholarBank@NUS Repository.
Abstract: Background aims. Interest in natural killer (NK) cell-based immunotherapy has resurged since new protocols for the purification and expansion of large numbers of clinical-grade cells have become available. Methods. We have successfully adapted a previously described NK expansion method that uses K562 cells expressing interleukin (IL)-15 and 4-1 BB Ligand (BBL) (K562-mb15-41BBL) to grow NK cells in novel gas-permeable static cell culture flasks (G-Rex). Results. Using this system we produced up to 19 × 109 functional NK cells from unseparated apheresis products, starting with 15 × 107 CD3 CD56 NK cells, within 810 days of culture. The G-Rex yielded a higher fold expansion of NK cells than conventional gas-permeable bags and required no cell manipulation or feeding during the culture period. We also showed that K562-mb15-41BBL cells up-regulated surface HLA class I antigen expression upon stimulation with the supernatants from NK cultures and stimulated alloreactive CD8 T cells within the NK cultures. However, these CD3 T cells could be removed successfully using the CliniMACS system. We describe our optimized NK cell cryopreservation method and show that the NK cells are viable and functional even after 12 months of cryopreservation. Conclusions. We have successfully developed a static culture protocol for large-scale expansion of NK cells in the gas permeable G-Rex system under good manufacturing practice (GMP) conditions. This strategy is currently being used to produce NK cells for cancer immunotherapy. © 2012 Informa Healthcare.
Source Title: Cytotherapy
ISSN: 14653249
DOI: 10.3109/14653249.2012.700767
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.