Please use this identifier to cite or link to this item: https://doi.org/10.1186/1743-422X-9-206
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dc.titleSf-PHB2, A new transcription factor, Drives WSSV Ie1 Gene Expression via a 12-bp DNA Element
dc.contributor.authorMa, G.
dc.contributor.authorYu, L.
dc.contributor.authorWang, Q.
dc.contributor.authorLiu, W.
dc.contributor.authorCui, Y.
dc.contributor.authorKwang, J.
dc.date.accessioned2016-07-08T09:28:06Z
dc.date.available2016-07-08T09:28:06Z
dc.date.issued2012
dc.identifier.citationMa, G., Yu, L., Wang, Q., Liu, W., Cui, Y., Kwang, J. (2012). Sf-PHB2, A new transcription factor, Drives WSSV Ie1 Gene Expression via a 12-bp DNA Element. Virology Journal 9 : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1743-422X-9-206
dc.identifier.issn1743422X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/125516
dc.description.abstractBackground: The WSSV immediate early gene ie1 is highly expressed throughout viral infection cycle and may play a central role in initiating viral replication during infection. Results: Here, a detailed characterization of the ie1 promoter was performed using deletion and mutation analyses to elucidate the role of the individual promoter motifs. Three results were obtained: 1) the ie1 promoter is a classical eukaryotic promoter that contains the initiator element (Inr) and TATA box responsible for the basal promoter activity; 2) mutation or truncation of a predicted Sp1 site decreased the level of promoter activity by about 3-fold, indicating that the Sp1 site is an important cis-element of the promoter; and 3) truncation of a 12-bp sequence that resides at -78/-67 of the ie1 promoter decreased the level of promoter activity by about 14-fold, indicating that the 12-bp motif is a critical upstream element of the ie1 promoter for binding of a strong transcription factor to drive the ie1 gene expression in the cells. Further, the 12-bp DNA binding protein was purified from the nuclear proteins of Sf9 cells using DNA affinity chromatography, and was identified as a homologue of the prohibitin2 protein (named as Sf-PHB2) using mass spectrometry. Furthermore, the DNA binding activity of Sf-PHB2 was verified using a super shift analysis. Conclusion: These results support that the Sf-PHB2 is a novel transcription factor that drives WSSV ie1 gene expression by binding to the 12-bp DNA element. © 2012 Ma et al.; licensee BioMed Central Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1186/1743-422X-9-206
dc.sourceScopus
dc.subject12-bp motif
dc.subjectie1 promoter
dc.subjectSf-PHB2
dc.subjectTranscription factor
dc.subjectWSSV
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1186/1743-422X-9-206
dc.description.sourcetitleVirology Journal
dc.description.volume9
dc.description.page-
dc.identifier.isiut000311756300001
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