Please use this identifier to cite or link to this item:
|Title:||Characterization of early host responses in adults with dengue disease||Authors:||Tolfvenstam, T.
|Issue Date:||2-Aug-2011||Citation:||Tolfvenstam, T., Lindblom, A., Schreiber, M.J., Ling, L., Chow, A., Ooi, E.E., Hibberd, M.L. (2011-08-02). Characterization of early host responses in adults with dengue disease. BMC Infectious Diseases 11 : -. ScholarBank@NUS Repository. https://doi.org/10.1186/1471-2334-11-209||Abstract:||Background: While dengue-elicited early and transient host responses preceding defervescence could shape the disease outcome and reveal mechanisms of the disease pathogenesis, assessment of these responses are difficult as patients rarely seek healthcare during the first days of benign fever and thus data are lacking.Methods: In this study, focusing on early recruitment, we performed whole-blood transcriptional profiling on denguevirus PCR positive patients sampled within 72 h of self-reported fever presentation (average 43 h, SD 18.6 h) and compared the signatures with autologous samples drawn at defervescence and convalescence and to control patients with fever of other etiology.Results: In the early dengue fever phase, a strong activation of the innate immune response related genes were seen that was absent at defervescence (4-7 days after fever debut), while at this second sampling genes related to biosynthesis and metabolism dominated. Transcripts relating to the adaptive immune response were over-expressed in the second sampling point with sustained activation at the third sampling. On an individual gene level, significant enrichment of transcripts early in dengue disease were chemokines CCL2 (MCP-1), CCL8 (MCP-2), CXCL10 (IP-10) and CCL3 (MIP-1α), antimicrobial peptide β-defensin 1 (DEFB1), desmosome/intermediate junction component plakoglobin (JUP) and a microRNA which may negatively regulate pro-inflammatory cytokines in dengue infected peripheral blood cells, mIR-147 (NMES1).Conclusions: These data show that the early response in patients mimics those previously described in vitro, where early assessment of transcriptional responses has been easily obtained. Several of the early transcripts identified may be affected by or mediate the pathogenesis and deserve further assessment at this timepoint in correlation to severe disease. © 2011 Tolfvenstam et al; licensee BioMed Central Ltd.||Source Title:||BMC Infectious Diseases||URI:||http://scholarbank.nus.edu.sg/handle/10635/125494||ISSN:||14712334||DOI:||10.1186/1471-2334-11-209|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
|2011-characterization_early_host_responses-published.pdf||443.03 kB||Adobe PDF|
checked on Mar 4, 2021
WEB OF SCIENCETM
checked on Feb 24, 2021
checked on Feb 27, 2021
checked on Feb 27, 2021
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.