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dc.titleA three-dimensional atlas of human dermal leukocytes, lymphatics, and blood vessels
dc.contributor.authorWang, X.-N.
dc.contributor.authorMcGovern, N.
dc.contributor.authorGunawan, M.
dc.contributor.authorRichardson, C.
dc.contributor.authorWindebank, M.
dc.contributor.authorSiah, T.-W.
dc.contributor.authorLim, H.-Y.
dc.contributor.authorFink, K.
dc.contributor.authorLi, J.L.Y.
dc.contributor.authorNg, L.G.
dc.contributor.authorGinhoux, F.
dc.contributor.authorAngeli, V.
dc.contributor.authorCollin, M.
dc.contributor.authorHaniffa, M.
dc.identifier.citationWang, X.-N., McGovern, N., Gunawan, M., Richardson, C., Windebank, M., Siah, T.-W., Lim, H.-Y., Fink, K., Li, J.L.Y., Ng, L.G., Ginhoux, F., Angeli, V., Collin, M., Haniffa, M. (2014). A three-dimensional atlas of human dermal leukocytes, lymphatics, and blood vessels. Journal of Investigative Dermatology 134 (4) : 965-974. ScholarBank@NUS Repository.
dc.description.abstractDendritic cells (DCs), macrophages (Mφ), and T cells are major components of the skin immune system, but their interstitial spatial organization is poorly characterized. Using four-channel whole-mount immunofluorescence staining of the human dermis, we demonstrated the three-dimensional distribution of CD31+ blood capillaries, LYVE-1+ lymphatics, discrete populations of CD11c+ myeloid DCs, FXIIIa+ Mφ, and lymphocytes. We showed phenotypic and morphological differences in situ between DCs and Mφ. DCs formed the first dermal cellular layer (0-20 μm beneath the dermoepidermal junction), Mφ were located deeper (40-60 μm), and CD3+ lymphocytes were observed throughout (0-60 μm). Below this level, DCs, T cells, and the majority of Mφ formed stable perivascular sheaths. Whole-mount imaging revealed the true extent of dermal leukocytes previously underestimated from cross-section views. The total area of apical dermis (0-30 μm) contained approximately 10-fold more myeloid DCs than the entire blood volume of an average individual. Surprisingly,
dc.description.sourcetitleJournal of Investigative Dermatology
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