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Title: A three-dimensional atlas of human dermal leukocytes, lymphatics, and blood vessels
Authors: Wang, X.-N.
McGovern, N.
Gunawan, M.
Richardson, C.
Windebank, M.
Siah, T.-W.
Lim, H.-Y. 
Fink, K.
Li, J.L.Y.
Ng, L.G.
Ginhoux, F.
Angeli, V. 
Collin, M.
Haniffa, M.
Issue Date: 2014
Citation: Wang, X.-N., McGovern, N., Gunawan, M., Richardson, C., Windebank, M., Siah, T.-W., Lim, H.-Y., Fink, K., Li, J.L.Y., Ng, L.G., Ginhoux, F., Angeli, V., Collin, M., Haniffa, M. (2014). A three-dimensional atlas of human dermal leukocytes, lymphatics, and blood vessels. Journal of Investigative Dermatology 134 (4) : 965-974. ScholarBank@NUS Repository.
Abstract: Dendritic cells (DCs), macrophages (Mφ), and T cells are major components of the skin immune system, but their interstitial spatial organization is poorly characterized. Using four-channel whole-mount immunofluorescence staining of the human dermis, we demonstrated the three-dimensional distribution of CD31+ blood capillaries, LYVE-1+ lymphatics, discrete populations of CD11c+ myeloid DCs, FXIIIa+ Mφ, and lymphocytes. We showed phenotypic and morphological differences in situ between DCs and Mφ. DCs formed the first dermal cellular layer (0-20 μm beneath the dermoepidermal junction), Mφ were located deeper (40-60 μm), and CD3+ lymphocytes were observed throughout (0-60 μm). Below this level, DCs, T cells, and the majority of Mφ formed stable perivascular sheaths. Whole-mount imaging revealed the true extent of dermal leukocytes previously underestimated from cross-section views. The total area of apical dermis (0-30 μm) contained approximately 10-fold more myeloid DCs than the entire blood volume of an average individual. Surprisingly,
Source Title: Journal of Investigative Dermatology
ISSN: 15231747
DOI: 10.1038/jid.2013.481
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