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|Title:||A three-dimensional atlas of human dermal leukocytes, lymphatics, and blood vessels||Authors:||Wang, X.-N.
|Issue Date:||2014||Citation:||Wang, X.-N., McGovern, N., Gunawan, M., Richardson, C., Windebank, M., Siah, T.-W., Lim, H.-Y., Fink, K., Li, J.L.Y., Ng, L.G., Ginhoux, F., Angeli, V., Collin, M., Haniffa, M. (2014). A three-dimensional atlas of human dermal leukocytes, lymphatics, and blood vessels. Journal of Investigative Dermatology 134 (4) : 965-974. ScholarBank@NUS Repository. https://doi.org/10.1038/jid.2013.481||Abstract:||Dendritic cells (DCs), macrophages (Mφ), and T cells are major components of the skin immune system, but their interstitial spatial organization is poorly characterized. Using four-channel whole-mount immunofluorescence staining of the human dermis, we demonstrated the three-dimensional distribution of CD31+ blood capillaries, LYVE-1+ lymphatics, discrete populations of CD11c+ myeloid DCs, FXIIIa+ Mφ, and lymphocytes. We showed phenotypic and morphological differences in situ between DCs and Mφ. DCs formed the first dermal cellular layer (0-20 μm beneath the dermoepidermal junction), Mφ were located deeper (40-60 μm), and CD3+ lymphocytes were observed throughout (0-60 μm). Below this level, DCs, T cells, and the majority of Mφ formed stable perivascular sheaths. Whole-mount imaging revealed the true extent of dermal leukocytes previously underestimated from cross-section views. The total area of apical dermis (0-30 μm) contained approximately 10-fold more myeloid DCs than the entire blood volume of an average individual. Surprisingly,||Source Title:||Journal of Investigative Dermatology||URI:||http://scholarbank.nus.edu.sg/handle/10635/125493||ISSN:||15231747||DOI:||10.1038/jid.2013.481|
|Appears in Collections:||Staff Publications|
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