Please use this identifier to cite or link to this item: https://doi.org/10.1038/emboj.2012.275
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dc.titleRUNX1 reshapes the epigenetic landscape at the onset of haematopoiesis
dc.contributor.authorLichtinger, M.
dc.contributor.authorIngram, R.
dc.contributor.authorHannah, R.
dc.contributor.authorMüller, D.
dc.contributor.authorClarke, D.
dc.contributor.authorAssi, S.A.
dc.contributor.authorLie-A-Ling, M.
dc.contributor.authorNoailles, L.
dc.contributor.authorVijayabaskar, M.S.
dc.contributor.authorWu, M.
dc.contributor.authorTenen, D.G.
dc.contributor.authorWesthead, D.R.
dc.contributor.authorKouskoff, V.
dc.contributor.authorLacaud, G.
dc.contributor.authorGöttgens, B.
dc.contributor.authorBonifer, C.
dc.date.accessioned2016-07-08T09:27:10Z
dc.date.available2016-07-08T09:27:10Z
dc.date.issued2012-11-14
dc.identifier.citationLichtinger, M., Ingram, R., Hannah, R., Müller, D., Clarke, D., Assi, S.A., Lie-A-Ling, M., Noailles, L., Vijayabaskar, M.S., Wu, M., Tenen, D.G., Westhead, D.R., Kouskoff, V., Lacaud, G., Göttgens, B., Bonifer, C. (2012-11-14). RUNX1 reshapes the epigenetic landscape at the onset of haematopoiesis. EMBO Journal 31 (22) : 4318-4333. ScholarBank@NUS Repository. https://doi.org/10.1038/emboj.2012.275
dc.identifier.issn02614189
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/125447
dc.description.abstractCell fate decisions during haematopoiesis are governed by lineage-specific transcription factors, such as RUNX1, SCL/TAL1, FLI1 and C/EBP family members. To gain insight into how these transcription factors regulate the activation of haematopoietic genes during embryonic development, we measured the genome-wide dynamics of transcription factor assembly on their target genes during the RUNX1-dependent transition from haemogenic endothelium (HE) to haematopoietic progenitors. Using a Runx1-/- embryonic stem cell differentiation model expressing an inducible Runx1 gene, we show that in the absence of RUNX1, haematopoietic genes bind SCL/TAL1, FLI1 and C/EBPβ and that this early priming is required for correct temporal expression of the myeloid master regulator PU.1 and its downstream targets. After induction, RUNX1 binds to numerous de novo sites, initiating a local increase in histone acetylation and rapid global alterations in the binding patterns of SCL/TAL1 and FLI1. The acquisition of haematopoietic fate controlled by Runx1 therefore does not represent the establishment of a new regulatory layer on top of a pre-existing HE program but instead entails global reorganization of lineage-specific transcription factor assemblies. © 2012 European Molecular Biology Organization.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1038/emboj.2012.275
dc.sourceScopus
dc.subjectCell fate decisions
dc.subjectendothelial-haematopoietic transition
dc.subjecthaematopoiesis
dc.subjectRUNX1
dc.subjecttranscriptional programming of chromatin
dc.typeArticle
dc.contributor.departmentMEDICINE
dc.description.doi10.1038/emboj.2012.275
dc.description.sourcetitleEMBO Journal
dc.description.volume31
dc.description.issue22
dc.description.page4318-4333
dc.description.codenEMJOD
dc.identifier.isiut000311157500008
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