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|Title:||Neurophysiological and immunohistochemical studies of IgG anti-GM1 monoclonal antibody on neuromuscular transmission: Effects in rat neuromuscular junctions||Authors:||Hotta, S.
|Keywords:||Anti-GM1 monoclonal antibody
Spontaneous muscle action potential
|Issue Date:||Feb-2014||Citation:||Hotta, S., Nagaoka, T., Taguchi, K., Nakatani, Y., Utsnomiya, I., Masuda, Y., Abe, K., Yuki, N. (2014-02). Neurophysiological and immunohistochemical studies of IgG anti-GM1 monoclonal antibody on neuromuscular transmission: Effects in rat neuromuscular junctions. Neurological Sciences 35 (2) : 205-213. ScholarBank@NUS Repository. https://doi.org/10.1007/s10072-013-1480-z||Abstract:||Guillain-Barré syndrome, which is a variant of acute inflammatory neuropathy, is associated with anti-GM1 antibodies and causes ataxia. We investigated the effects of IgG anti-GM1 monoclonal antibody (IgG anti-GM1 mAb) on spontaneous muscle action potentials in a rat spinal cord-muscle co-culture system and the localization of IgG anti-GM1 mAb binding in the rat hemi-diaphragm. The frequency of spontaneous muscle action potentials in innervated muscle cells was acutely inhibited by IgG anti-GM1 mAb. When cultures were pretreated with GM2 synthase antisense oligodeoxynucleotide, IgG anti-GM1 mAb failed to inhibit spontaneous muscle action potentials, demonstrating the importance of the GM1 epitope in the action of IgG anti-GM1 mAb. Immunohistochemistry of rat hemi-diaphragm showed that IgG anti-GM1 mAb binding overlapped with neurofilament 200 (NF200) antibodies staining, but not α-bungarotoxin (α-BuTx) staining, demonstrating that IgG anti-GM1 mAb was localized at the presynaptic nerve terminal. IgG anti-GM1 mAb binding overlapped with syntaxin antibody and S-100 antibody in the nerve terminal. After collagenase treatment, IgG anti-GM1 mAb and NF200 antibodies did not show staining, but α-BuTx selectively stained the hemi-diaphragm. IgG anti-GM1 mAb binds to the presynaptic nerve terminal of neuromuscular junctions. Therefore, we suggest that the inhibitory effect of IgG anti-GM1 mAb on spontaneous muscle action potentials is related to the GM1 epitope in presynaptic motor nerve terminals at the NMJs. © 2013 Springer-Verlag Italia.||Source Title:||Neurological Sciences||URI:||http://scholarbank.nus.edu.sg/handle/10635/125420||ISSN:||15901874||DOI:||10.1007/s10072-013-1480-z|
|Appears in Collections:||Staff Publications|
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