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Title: Effect of 710nm visible light irradiation on neurite outgrowth in primary rat cortical neurons following ischemic insult
Authors: Choi, D.-H.
Lee, K.-H.
Kim, J.-H.
Kim, M.Y.
Lim, J.H. 
Lee, J.
Keywords: 710nm light irradiation
Cortical neurons
Neurite outgrowth
Oxygen-glucose deprivation
Synaptic proteins
Issue Date: 1-Jun-2012
Citation: Choi, D.-H., Lee, K.-H., Kim, J.-H., Kim, M.Y., Lim, J.H., Lee, J. (2012-06-01). Effect of 710nm visible light irradiation on neurite outgrowth in primary rat cortical neurons following ischemic insult. Biochemical and Biophysical Research Communications 422 (2) : 274-279. ScholarBank@NUS Repository.
Abstract: Objective: We previously reported that 710. nm Light-emitting Diode (LED) has a protective effect through cellular immunity activation in the stroke animal model. However, whether LED directly protects neurons suffering from neurodegeneration was entirely unknown. Therefore, we sought to determine the effects of 710. nm visible light irradiation on neuronal protection and neuronal outgrowth in an in vitro stroke model. Materials & methods: Primary cultured rat cortical neurons were exposed to oxygen-glucose deprivation (OGD) and reoxygenation and normal conditions. An LED array with a peak wavelength of 710nm was placed beneath the covered culture dishes with the room light turned off and were irradiated accordingly. LED treatments (4min at 4J/cm 2 and 50mW/cm 2) were given once to four times within 8h at 2h intervals for 7days. Mean neurite density, mean neurite diameter, and total fiber length were also measured after microtubule associated protein 2 (MAP2) immunostaining using the Axio Vision program. Synaptic marker expression and MAPK activation were confirmed by Western blotting. Results: Images captured after MAP2 immunocytochemistry showed significant (p
Source Title: Biochemical and Biophysical Research Communications
ISSN: 0006291X
DOI: 10.1016/j.bbrc.2012.04.147
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