Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/125164
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dc.titleMETHODOLOGY DEVELOPMENT FOR ENVIRONMENTAL MICROBIOLOGY: MEDIUM FORMULATION AND SURFACE CHARGE ANALYSIS
dc.contributor.authorNG WENFA
dc.date.accessioned2016-06-20T18:00:07Z
dc.date.available2016-06-20T18:00:07Z
dc.date.issued2012-04-03
dc.identifier.citationNG WENFA (2012-04-03). METHODOLOGY DEVELOPMENT FOR ENVIRONMENTAL MICROBIOLOGY: MEDIUM FORMULATION AND SURFACE CHARGE ANALYSIS. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/125164
dc.description.abstractAnalytical techniques afford scientists the tools to see further and deeper into their subjects of investigation. This thesis attempted to develop new and improved methods in the areas of cultivation of microorganisms from environmental water samples and bacterial surface charge analysis. Firstly, a colourless agar medium was formulated in an attempt to solve the “Great Plate Count Anomaly” problem. Secondly, as there is no consensus on the type of wash buffer for the preparation of bacterial biomass for surface charge analysis, a systematic evaluation of the effectiveness of various wash buffers in removing nonspecifically adsorbed ions and charged molecules from the bacterial cell surface was performed.
dc.language.isoen
dc.subjectMedium formulation, Colourless agar, As-yet-not-cultivated problem, Surface charge analysis
dc.typeThesis
dc.contributor.departmentCHEMICAL & BIOMOLECULAR ENGINEERING
dc.contributor.supervisorKang Zhou
dc.contributor.supervisorKus Hidajat
dc.description.degreeMaster's
dc.description.degreeconferredMASTER OF ENGINEERING (FOE)
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Master's Theses (Open)

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