Please use this identifier to cite or link to this item: https://doi.org/10.1093/hmg/ddu026
Title: Thiol peroxidases ameliorateLRRK2mutant-induced mitochondrial and dopaminergic neuronal degeneration in Drosophila
Authors: Angeles, D.C.
Ho, P.
Chua, L.L.
Wang, C. 
Yap, Y.W.
Ng, C.
Zhou, Z.D.
Lim, K.-L. 
Wszolek, Z.K.
Wang, H.Y. 
Tan, E.K. 
Issue Date: 2014
Citation: Angeles, D.C., Ho, P., Chua, L.L., Wang, C., Yap, Y.W., Ng, C., Zhou, Z.D., Lim, K.-L., Wszolek, Z.K., Wang, H.Y., Tan, E.K. (2014). Thiol peroxidases ameliorateLRRK2mutant-induced mitochondrial and dopaminergic neuronal degeneration in Drosophila. Human Molecular Genetics 23 (12) : 3157-3165. ScholarBank@NUS Repository. https://doi.org/10.1093/hmg/ddu026
Abstract: Mutations in leucine-rich repeat kinase 2 (LRRK2) are common causes of familial Parkinson's disease (PD). LRRK2 has been shown to bind peroxiredoxin-3 (PRDX3), the most important scavenger of hydrogen peroxide in the mitochondria, in vitro. Here, we examined the interactions of LRRK2 and PRDX3 in Drosophila models by crossing transgenic LRRK2 and PRDX3 flies. As proof of principle experiments, we subsequently challenged LRRK2 and LRRK2/PRDX3 flies with a peroxidase mimic, Ebselen. We demonstrated that co-expression of PRDX3 with the LRRK2 kinase mutant G2019S in bigenic Drosophila ameliorated the G2019S mutant-induced reduction in peroxidase capacity, loss of dopaminergic neurons, shortened lifespan and mitochondrial defects of flight muscles inmonogenic flies expressing theG2019Salone. Challenges with Ebselenrecapitulated similar rescue of these phenotypic features in mutant-expressing Drosophila. The peroxidase mimic preserved neuronal and mitochondrial and neuronal integrity and improved mobility and survival in mutant-expressing Drosophila. Taken together, our study provides the first in vivo evidence to suggest that phosphoinhibition of endogenous peroxidases could be amechanism in LRRK2-induced oxidant-mediated neurotoxicity.Our therapeutic experiments also highlight the potential of thiol peroxidases as neuroprotective agents in PD patients carrying LRRK2 mutations. © The Author 2014. Published by Oxford University Press. All rights reserved.
Source Title: Human Molecular Genetics
URI: http://scholarbank.nus.edu.sg/handle/10635/124894
ISSN: 14602083
DOI: 10.1093/hmg/ddu026
Appears in Collections:Staff Publications
Elements

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
ddu026.pdf927.83 kBAdobe PDF

OPEN

PublishedView/Download

SCOPUSTM   
Citations

19
checked on Oct 10, 2019

WEB OF SCIENCETM
Citations

19
checked on Oct 3, 2019

Page view(s)

105
checked on Oct 11, 2019

Download(s)

2
checked on Oct 11, 2019

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.