Please use this identifier to cite or link to this item:
|Title:||Kinetic profiling of the c-Myc transcriptome and bioinformatic analysis of repressed gene promoters||Authors:||Yap, C.-S.
|Issue Date:||1-Jul-2011||Citation:||Yap, C.-S., Peterson, A.L., Castellani, G., Sedivy, J.M., Neretti, N. (2011-07-01). Kinetic profiling of the c-Myc transcriptome and bioinformatic analysis of repressed gene promoters. Cell Cycle 10 (13) : 2184-2196. ScholarBank@NUS Repository. https://doi.org/10.4161/cc.10.13.16249||Abstract:||Mammalian c-Myc is a member of a small family of three related proto-oncogenic transcription factors. c-Myc has an unusually broad array of regulatory functions, which include roles in cell cycle and apoptosis, a variety of metabolic functions, cell differentiation, senescence and stem cell maintenance. c-Myc modulates the expression of a very large number of genes, but the magnitude of the majority of the regulatory effects is only 2-fold or less. c-Myc can both activate and repress the promoters of its target genes. Identification of genes directly regulated by c-Myc has been an enduring question in the field. We report here microarray expression profiling of a high resolution time course of c-Myc induction, using fibroblast cells in which c-Myc activity can be modulated from null to physiological. The c-Myc transcriptome data set presented is the largest reported to date with 4,186 differentially regulated genes (1,826 upregulated, 2,360 downregulated, 1% FDR). The gene expression patterns fit well with the known biological functions of c-Myc. We describe several novel findings and present tools for further data mining. Although the mechanisms of transcriptional activation by c-Myc are well understood, how c-Myc represses an even greater number of genes remains incompletely described. One mechanism involves the binding of c-Myc to other, positively acting transcription factors and interfering with their activities. We identified rapid-response genes likely to be direct c-Myc targets and analyzed the promoters of the repressed genes to identify transcription factors that could be targets of c-Myc repression. © 2011 Landes Bioscience.||Source Title:||Cell Cycle||URI:||http://scholarbank.nus.edu.sg/handle/10635/124750||ISSN:||15384101||DOI:||10.4161/cc.10.13.16249|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 20, 2020
WEB OF SCIENCETM
checked on Oct 20, 2020
checked on Oct 17, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.