Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-62703-239-1-8
DC FieldValue
dc.titleColorimetric detection of senescence-associated β galactosidase
dc.contributor.authorItahana, K.
dc.contributor.authorItahana, Y.
dc.contributor.authorDimri, G.P.
dc.date.accessioned2016-06-01T10:28:02Z
dc.date.available2016-06-01T10:28:02Z
dc.date.issued2013
dc.identifier.citationItahana, K.,Itahana, Y.,Dimri, G.P. (2013). Colorimetric detection of senescence-associated β galactosidase. Methods in Molecular Biology 965 : 143-156. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/978-1-62703-239-1-8" target="_blank">https://doi.org/10.1007/978-1-62703-239-1-8</a>
dc.identifier.isbn9781627032384
dc.identifier.issn10643745
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/124746
dc.description.abstractMost normal human cells have a finite replicative capacity and eventually undergo cellular senescence, whereby cells cease to proliferate. Cellular senescence is also induced by various stress signals, such as those generated by oncogenes, DNA damage, hyperproliferation, and an oxidative environment. Cellular senescence is well established as an intrinsic tumor suppressive mechanism. Recent progress concerning senescence research has revealed that cellular senescence occurs in vivo and that, unexpectedly, it has a very complex role in tissue repair, promoting tumor progression and aging via the secretion of various cytokines, growth factors, and enzymes. Therefore, the importance of biomarkers for cellular senescence has greatly increased. In 1995, we described the "senescence-associated β galactosidase" (SA-βgal) biomarker, which conveniently identifies individual senescent cells in vitro and in vivo. Here, we describe an updated protocol for the detection of cell senescence based on this widely used biomarker, which contributed to recent advances in senescence, aging and cancer research. We provide an example of detecting SA-βgal together with other senescence markers and a proliferation marker, EdU, in single cells. © Springer Science+Business Media New York 2013.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/978-1-62703-239-1-8
dc.sourceScopus
dc.subjectAging
dc.subjectBiomarker
dc.subjectCellular senescence
dc.subjectEdU labeling
dc.subjectImmunostaining
dc.subjectSA-βgal
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1007/978-1-62703-239-1-8
dc.description.sourcetitleMethods in Molecular Biology
dc.description.volume965
dc.description.page143-156
dc.identifier.isiutNOT_IN_WOS
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