Please use this identifier to cite or link to this item: https://doi.org/10.1002/jmv.21721
Title: The prevalence of antibodies to adenovirus serotype 5 in an adult indian population and implications for adenovirus vector vaccines
Authors: Pilankatta, R.
Chawla, T. 
Khanna, N.
Swaminathan, S.
Keywords: Adenoviral vaccine
Adenovirus
CAR
Fc receptor
Prior adenovirus immunity
Issue Date: 2010
Citation: Pilankatta, R., Chawla, T., Khanna, N., Swaminathan, S. (2010). The prevalence of antibodies to adenovirus serotype 5 in an adult indian population and implications for adenovirus vector vaccines. Journal of Medical Virology 82 (3) : 407-414. ScholarBank@NUS Repository. https://doi.org/10.1002/jmv.21721
Abstract: In vivo gene delivery using human adenovirus serotype 5 (AdV5) vectors is being explored for vaccination purposes. The presence of anti-AdV5 antibodies in human serum arising from natural exposure to AdV5 can interfere potentially with and compromise the efficacy of rAdV5-based vaccine vectors. In this report, a collection of 114 sera from healthy adult Indian blood donors was analyzed for the presence of anti-AdV5 antibodies, using an AdV5 vector encoding the green fluorescent protein (GFP) to monitor the presence of anti-AdV5 neutralizing antibodies in human sera based on their ability to block virus entry into HeLa cells which express the Coxsackievirus-and-Adenovirus Receptor (CAR). In this assay all samples tested were positive for anti-AdV5 antibodies, with titers varying over a very wide range. It was also observed that these antibodies facilitated the uptake of the reporter AdV5 vector into the monocytic cell line U937 which does not express CAR, but expresses Fc receptors (FcRs) instead. These observations have implications for rAdV5-based vaccine development. © 2010 Wiley-Liss, Inc.
Source Title: Journal of Medical Virology
URI: http://scholarbank.nus.edu.sg/handle/10635/124741
ISSN: 01466615
DOI: 10.1002/jmv.21721
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