Please use this identifier to cite or link to this item: https://doi.org/10.1126/science.1240617
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dc.titleSingle-cell DNA-methylation analysis reveals epigenetic chimerism in preimplantation embryos
dc.contributor.authorLorthongpanich, C.
dc.contributor.authorCheow, L.
dc.contributor.authorBalu, S.
dc.contributor.authorQuake, S.R.
dc.contributor.authorKnowles, B.B.
dc.contributor.authorBurkholder, W.F.
dc.contributor.authorSolter, D.
dc.contributor.authorMesserschmidt, D.M.
dc.date.accessioned2016-06-01T10:24:59Z
dc.date.available2016-06-01T10:24:59Z
dc.date.issued2013
dc.identifier.citationLorthongpanich, C., Cheow, L., Balu, S., Quake, S.R., Knowles, B.B., Burkholder, W.F., Solter, D., Messerschmidt, D.M. (2013). Single-cell DNA-methylation analysis reveals epigenetic chimerism in preimplantation embryos. Science 341 (6150) : 1110-1112. ScholarBank@NUS Repository. https://doi.org/10.1126/science.1240617
dc.identifier.issn00368075
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/124656
dc.description.abstractEpigenetic alterations are increasingly recognized as causes of human cancers and disease. These aberrations are likely to arise during genomic reprogramming in mammalian preimplantation embryos, when their epigenomes are most vulnerable. However, this process is only partially understood because of the experimental inaccessibility of early-stage embryos. Here, we introduce a methodologic advance, probing single cells for various DNA-methylation errors at multiple loci, to reveal failed maintenance of epigenetic mark results in chimeric mice, which display unpredictable phenotypes leading to developmental arrest. Yet we show that mouse pronuclear transfer can be used to ameliorate such reprogramming defects. This study not only details the epigenetic reprogramming dynamics in early mammalian embryos but also suggests diagnostic and potential future therapeutic applications.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1126/science.1240617
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentDUKE-NUS GRADUATE MEDICAL SCHOOL S'PORE
dc.description.doi10.1126/science.1240617
dc.description.sourcetitleScience
dc.description.volume341
dc.description.issue6150
dc.description.page1110-1112
dc.description.codenSCIEA
dc.identifier.isiut000323933100044
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