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Title: Single-cell DNA-methylation analysis reveals epigenetic chimerism in preimplantation embryos
Authors: Lorthongpanich, C.
Cheow, L.
Balu, S.
Quake, S.R.
Knowles, B.B.
Burkholder, W.F.
Solter, D. 
Messerschmidt, D.M.
Issue Date: 2013
Citation: Lorthongpanich, C., Cheow, L., Balu, S., Quake, S.R., Knowles, B.B., Burkholder, W.F., Solter, D., Messerschmidt, D.M. (2013). Single-cell DNA-methylation analysis reveals epigenetic chimerism in preimplantation embryos. Science 341 (6150) : 1110-1112. ScholarBank@NUS Repository.
Abstract: Epigenetic alterations are increasingly recognized as causes of human cancers and disease. These aberrations are likely to arise during genomic reprogramming in mammalian preimplantation embryos, when their epigenomes are most vulnerable. However, this process is only partially understood because of the experimental inaccessibility of early-stage embryos. Here, we introduce a methodologic advance, probing single cells for various DNA-methylation errors at multiple loci, to reveal failed maintenance of epigenetic mark results in chimeric mice, which display unpredictable phenotypes leading to developmental arrest. Yet we show that mouse pronuclear transfer can be used to ameliorate such reprogramming defects. This study not only details the epigenetic reprogramming dynamics in early mammalian embryos but also suggests diagnostic and potential future therapeutic applications.
Source Title: Science
ISSN: 00368075
DOI: 10.1126/science.1240617
Appears in Collections:Staff Publications

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