Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/122332
Title: AMYLOID PRECURSOR PROTEIN SIGNALING IN PHYSIOLOGY AND ALZHEIMER?S DISEASE
Authors: JOANITA BINTE JASMEN
Keywords: Amyloid precursor protein, Amyloid precursor protein intracellular domain, Alzheimer’s disease, chromatin immunoprecipitation, microarray
Issue Date: 19-Dec-2014
Citation: JOANITA BINTE JASMEN (2014-12-19). AMYLOID PRECURSOR PROTEIN SIGNALING IN PHYSIOLOGY AND ALZHEIMER?S DISEASE. ScholarBank@NUS Repository.
Abstract: 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
URI: http://scholarbank.nus.edu.sg/handle/10635/122332
Appears in Collections:Ph.D Theses (Open)

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