Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/121046
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dc.titleROLES OF ALPHA-ACTININ IN THE REGULATION OF MITOTIC SPINDLE ORGANIZATION IN MAMMALIAN CELLS
dc.contributor.authorVINAYAKA SRINIVAS
dc.date.accessioned2015-09-23T18:19:34Z
dc.date.available2015-09-23T18:19:34Z
dc.date.issued2013-01-24
dc.identifier.citationVINAYAKA SRINIVAS (2013-01-24). ROLES OF ALPHA-ACTININ IN THE REGULATION OF MITOTIC SPINDLE ORGANIZATION IN MAMMALIAN CELLS. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/121046
dc.description.abstractCytokinesis is the final step of cell division and is a tightly regulated spatio-temporal process. Proper positioning of the cleavage furrow requires microtubules including dynamic astral microtubules and stable equatorial microtubules. However, how these two populations of microtubules are regulated in a time and space dependent manner is not clearly understood. Earlier we had shown that over-expression of a-actinin an actin cross-linking protein increases the cortical stiffness of the cell and depletion of a-actinin leads to the formation of ectopic furrows. So, we next investigated the effect of depletion of a-actinin on microtubule organization during cytokinesis. Interesting we found depletion of a-actinin lead to the aberrant localization of centralspindlin, but not aurora B or PRC1, to the tips of astral microtubules which caused the stabilization of the astral microtubules. Depletion of a-actinin also caused impaired organization of midzone microtubules and relocation of aurora B from chromosomes to these microtubules, suggesting that ectopic recruitment of MKLP1 leads to a failure of assembly of midzone microtubules. Our findings unveil an unexpected yet crucial role for an actin crosslinking protein in the regulation of the localization of the microtubule-associated cytokinetic regulator.
dc.language.isoen
dc.subjectα-actinin, Cell division, Cleavage furrow positioning, Centralspindlin, Microtubule
dc.typeThesis
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.supervisorMOHAN K BALASUBRAMANIAN
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
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