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Title: Histochemical and immunohistochemical localisation of nitrergic neuronal and non-neuronal cells in the bursa of Fabricius of the chicken
Authors: Ali, S.-M.
Chan, A.-S. 
Leong, S.-K. 
Keywords: Bursa of Fabricius
Neuron-specific enolase
Nicotinamide adenine dinucleotide hydrogen phosphate-diaphorase (NADPH-d)
Nitric oxide synthase
Issue Date: 1996
Citation: Ali, S.-M., Chan, A.-S., Leong, S.-K. (1996). Histochemical and immunohistochemical localisation of nitrergic neuronal and non-neuronal cells in the bursa of Fabricius of the chicken. Cell and Tissue Research 285 (2) : 273-279. ScholarBank@NUS Repository.
Abstract: The bursa of Fabricius of 24 chickens, 2-4 weeks old, was investigated to determine the distribution of nicotinamide adenine dinucleotide hydrogen phosphate-diaphorase (NADPH-d) staining and nitric oxide synthase (NOS) antigen, by use of NADPH-d histochemistry and NOS immunohistochemistry, respectively. NADPH-d reaction product was localised in neuronal cell bodies and nerve fibres. The stained cell bodies were predominantly in the different bursal compartments; some occurred in the wall and between the lymphoid follicles, and some in the immediate vicinity of blood vessels. Stained nerve fibres travelled mostly with blood vessels, but many were also observed in the connective tissue of the bursa, independent of blood vessels, and some in contact with the lymphoid follicle and interfollicular epithelium. In addition to neuronal profiles, the interfollicular epithelium of the plicae, and the vascular endothelium were densely stained, and lymphocytes were moderately labelled. NOS immunoreactivity was found in neuron-like cells and fibres which were confirmed to be neuronal in adjacent sections stained with antibodies raised against neuron-specific enolase. It was also detected in interfollicular epithelium and lymphocytes but not in vascular endothelium. The distribution patterns of NADPH-d and NOS suggest that nitric oxide (NO) may play a role in the regulation of the secretory activity of interfollicular epithelium and the blood flow through the bursa.
Source Title: Cell and Tissue Research
ISSN: 0302766X
DOI: 10.1007/s004410050644
Appears in Collections:Staff Publications

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