STUDY THE EFFECT OF TOPOGRAPHY DESIGN ON CELLULAR BEHAVIOUR

Abstract

ADVANCEMENT IN NANOTECHNOLOGY ENABLED RESEARCHERS TO REVEAL THE EFFECT OF TOPOGRAPHY ON CELLULAR BEHAVIOURS. HOWEVER, THE KEY TOPOGRAPHICAL PARAMETERS IN MODULATING CELLULAR BEHAVIOUR ARE STILL UNCLEAR. DIFFERENT TOPOGRAPHICAL DESIGN PARAMETERS WERE INVESTIGATED ON CELL BEHAVIOURS RELEVANT TOWARDS VASCULAR ENGINEERING AND DRUG AND GENE DELIVERY. FIRSTLY, PATTERNING OF PVA HYDROGEL VIA NANOIMPRINT LITHOGRAPHY AND SOFT LITHOGRAPHY WAS ACHIEVED. TOPOGRAPHIES IMPROVED HUVEC ADHESION ON PVA HYDROGEL IN VITRO AND IN VIVO. THIS WOULD ENHANCE ENDOTHELIALISATION RESPONSES ON PVA HYDROGEL TOWARDS VASCULAR GRAFT APPLICATIONS. SECONDLY, HIGH-THROUGHPUT SCREENING OF TOPOGRAPHY USING MULTI-ARCHITECTURE (MARC) CHIP IDENTIFIED SEVERAL TOPOGRAPHICAL DESIGNS SUCH AS TOPOGRAPHY WITH CURVATURE AND HIERARCHICAL TOPOGRAPHY THAT MODULATE HUVEC ADHESION, PROLIFERATION RATE AND TRANSFECTION EFFICIENCY. THIRDLY, TOPOGRAPHY FEATURE SIZE ALSO MODULATE INTERNALIZATION OF FITC-DEXTRAN AND TRANSFECTION EFFICIENCY DI