Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/120580
Title: FUNCTION OF THE MYCOBACTERIUM-SPECIFIC LOOP OF THE GAMMA SUBUNIT OF ATP SYNTHASE
Authors: MANUEL ADRIAN SUTER
Keywords: Tuberculosis, Mycobacterium tuberculosis, Mycobacterium smegmatis, Y subunit, ATP synthase, Recombineering
Issue Date: 22-Dec-2014
Citation: MANUEL ADRIAN SUTER (2014-12-22). FUNCTION OF THE MYCOBACTERIUM-SPECIFIC LOOP OF THE GAMMA SUBUNIT OF ATP SYNTHASE. ScholarBank@NUS Repository.
Abstract: THE MULTI-SUBUNIT ENZYME COMPLEX F1FO ATP SYNTHASE IS A KEY ENZYME IN CELLULAR ENERGY METABOLISM. IN MYCOBACTERIUM THIS ENZYME CONSISTS OF A MEMBRANE-EMBEDDED FO DOMAIN AND A CYTOSOLIC F1 PART. SUBUNIT ? IS PART OF THE F1 DOMAIN AND LINKS IT WITH THE FO DOMAIN. IT CONTAINS A UNIQUE AMINO ACID SEQUENCE INSERTION, ?166-179, WHICH IS NEITHER PRESENT IN EUKARYOTES NOR IN OTHER PROKARYOTES. THE FUNCTION OF THIS SHORT PEPTIDE INSERTION IS UNKNOWN. USING RECOMBINEERING TECHNIQUE, I CREATED A MUTATION IN THE M. SMEGMATIS MC2 155 GENE ATPG CODING FOR SUBUNIT ? OF F1FO ATP SYNTHASE LACKING A 42 BP DNA SEQUENCE CODING FOR THIS LOOP. COMPARATIVE GROWTH PHYSIOLOGY STUDIES ON A WHOLE CELL LEVEL UNDER AEROBIC AND ANAEROBIC CONDITIONS DID NOT REVEAL ANY DIFFERENCES BETWEEN M. SMEGMATIS WILD TYPE AND ?ATPG166-179 MUTANT.
URI: http://scholarbank.nus.edu.sg/handle/10635/120580
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
SuterMA.pdf1.37 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

155
checked on Oct 14, 2021

Download(s)

48
checked on Oct 14, 2021

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.