Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/120493
Title: TREATMENT OF ISCHEMIC STROKE BY TARGETING SODIUM-POTASSIUM-ATPASE AND HYDROGEN SULFIDE
Authors: XINGZHOU ZHANG
Keywords: Na+/K+ ATPase, DR-region specific antibody, hydrogen sulfide, ADTOH-danshensu, ischemic stroke, neuroprotection
Issue Date: 23-Jan-2015
Citation: XINGZHOU ZHANG (2015-01-23). TREATMENT OF ISCHEMIC STROKE BY TARGETING SODIUM-POTASSIUM-ATPASE AND HYDROGEN SULFIDE. ScholarBank@NUS Repository.
Abstract: EVIDENCES SUGGEST THAT MODULATING NA-K-ATPASE (NKA) AND HYDROGEN SULFIDES ARE POTENTIAL THERAPEUTIC STRATEGIES OF ISCHEMIC STROKE. WE DEVELOPED A SITE-SPECIFIC ANTIBODY (DR-AB), WHICH CAN ACTIVATE NKA WHEN IT BINDS TO AN EXTRACELLULAR REGION 897DVEDSYGQQWTYEQR911 (DR REGION) OF NKA. WE ALSO SYNTHESIZED A HYDROGEN SULFIDE RELEASING COMPOUND ADTOH-DANSHENSU (ADTOH-DSS). I EXAMINED THE EFFECTS OF THESE TWO POTENTIAL NEUROPROTECTANTS IN ISCHEMIC STROKE USING BOTH IN VITRO AND IN VIVO MODELS. MY RESULTS SHOWED THAT BOTH PRE- AND POST-TREATMENT WITH DR-AB HAD PROTECTIVE EFFECTS IN MICE SUBJECTED TO TRANSIENT MIDDLE CEREBRAL ARTERY OCCLUSION. THESE BENEFICIAL EFFECTS WERE ACCOMPANIED WITH RESTORED NKA ACTIVITY, IMPROVED CA2+ HANDLING, DECREASED APOPTOSIS AND SUPPRESSED INFLAMMATION. ADTOH-DSS PRETREATMENT PROTECTED SH-SY5Y CELLS FROM OGD/R-INDUCED INJURIES BUT FAILED TO EXERT PROTECTIVE EFFECTS IN TMCAO MICE. IN SUMMARY, THE CURRENT STUDY SHOWED THAT STIMULATING NKA WITH THE DR-SPECIFIC ANT
URI: http://scholarbank.nus.edu.sg/handle/10635/120493
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