PROTEOMIC STUDY OF GONADOTROPIN- RELEASING HORMONE TREATED GONADOTROPE CELL LINE

Abstract

Gonadotropin-releasing hormone (GnRH) is the first key hormone of reproduction. An understanding of the GnRH signaling mechanisms is crucial for establishing interactive signaling map, pointing to targets for new drugs. To explore new pathways and new regulatory mechanisms initiated by GnRH, we performed high-throughput proteomic studies in gonadotrope cell line. In ICAT study, we proposed a model showing the crosstalk between GnRH signaling and Wnt signaling. In iTRAQ study, we identified a number of differentially regulated proteins with novel functional roles, suggesting multifunctional roles of GnRH in the regulation of gonadotropins. Furthermore, we identified several potential Ca2+ signaling regulators, which were differentially regulated by GnRH pulse frequency, suggesting the crucial role of Ca2+ signaling in decoding the GnRH pulse frequency. Finally we proved that ACTN4 is a negative regulator of FSHß gene transcription, and the CaM-like domain is required for this function.