Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/119870
Title: GENE EDITING OF DND BY TALENS IN MEDAKA EMBRYOS AND ITS ROLE IN PGC FORMATION
Authors: WANG TIANSU
Keywords: dead end, dnd, gene editing, medaka, PGC, TALEN
Issue Date: 23-Jan-2015
Citation: WANG TIANSU (2015-01-23). GENE EDITING OF DND BY TALENS IN MEDAKA EMBRYOS AND ITS ROLE IN PGC FORMATION. ScholarBank@NUS Repository.
Abstract: TARGETED GENE EDITING (GE) IS POWERFUL FOR GENERATING GENETIC ALTERATIONS IN ANIMAL GENOMES. IN THIS THESIS, PROCEDURES AND PARAMETERS OF TALEN-MEDIATED GE OF A GERM LINE-SPECIFIC GENE DEAD END (DND) IN THE FISH MEDAKA WERE EXAMINED. MEDAKA EMBRYOS TOLERATED HIGH DOSAGES OF TALEN-MRNA AND EXHIBITED INCREASING GE EFFICIENCIES. THIS DOSAGE PRODUCED ~24% EFFICIENCY FOR SOMATIC GE. THESE DATA DEMONSTRATE THAT TALEN IS PROFICIENT FOR SOMATIC AND GERM LINE GE IN MEDAKA EMBRYOS. DND WAS FIRST IDENTIFIED IN ZEBRAFISH, ENCODING AN RNA-BINDING PROTEIN ESSENTIAL FOR THE SURVIVAL OF PRIMORDIAL GERM CELLS (PGCS). DND IS ALSO WELL CONSERVED IN MANY OTHER VERTEBRATES. IN THIS THESIS, THE ROLE OF DND IN MEDAKA GERM CELL DEVELOPMENT WAS ANALYZED BY TALEN-MEDIATED GE, DEMONSTRATING AN ESSENTIAL ROLE OF DND IN THE FORMATION AND SURVIVAL OF PGCS AND POST-MIGRATORY GERM CELL DEVELOPMENT.
URI: http://scholarbank.nus.edu.sg/handle/10635/119870
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