CHEMICAL ANALYSIS AND POTENTIAL APPLICATION OF CORN BIO-ETHANOL CO-PRODUCTS

Abstract

AIMING AT MAXIMIZING THE VALUE OF THE SURPLUS ETHANOL CO-PRODUCTS, THIS THESIS PROPOSED A PROCESS FROM WHICH POTENTIAL INGREDIENTS FOR DIETARY SUPPLEMENTS, COATING MATERIALS, AND SEASONING CAN BE OBTAINED. THE EXTRACTION WITH ETHYL ACETATE AND ALCOHOL FROM SEVEN TYPES OF BIO-ETHANOL CO-PRODUCTS (1, LIQUEFIED CORN MASH; 2, BEER; 3, WHOLE STILLAGE; 4, SYRUP; 5, CORN OIL; 6, WDGS; 7, DDGS) WERE ANALYZED FOR PHENOLIC ACIDS, A-TOCOPHEROL, AND CAROTENOIDS AND THEIR CHANGES DURING DRY GRINDING PROCESS WERE FOUND. THE RESIDUES FROM ABOVE EXTRACTION WERE FURTHER EXTRACTED WITH AQUEOUS ALCOHOL TO OBTAIN ZEIN, WHICH SHOWED CLOSE MOLECULAR PROFILES WITH COMMERCIAL ZEIN. THE RESULTING RESIDUES WERE FURTHER EXTRACTED FOR THE NUCLEOTIDES AND PROTEINS. THE NUCLEIC ACIDS WERE HYDROLYZED BY NUCLEASE P1 TO OBTAIN 5'-GMP, 5?-AMP (CAN BE DEAMINIZED TO 5'-IMP). FURTHERMORE, THE PROTEINS WERE HYDROLYZED INTO AMINO ACIDS AND PEPTIDES BY PROTAMEX AND FLAVOURZYME AND OBTAINED GLUTAMIC ACID.