Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/119595
DC FieldValue
dc.titleIDENTIFICATION AND EXPRESSION OF BOULE, DAZL AND VASA IN GERM CELLS OF THE ASIAN SEABASS
dc.contributor.authorMANALI AGARA DWARAKANATH
dc.date.accessioned2015-05-12T18:00:12Z
dc.date.available2015-05-12T18:00:12Z
dc.date.issued2014-12-03
dc.identifier.citationMANALI AGARA DWARAKANATH (2014-12-03). IDENTIFICATION AND EXPRESSION OF BOULE, DAZL AND VASA IN GERM CELLS OF THE ASIAN SEABASS. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/119595
dc.description.abstractTHE ASIAN SEABASS (LATES CALCARIFER) IS A HERMAPHRODITIC FISH, WHICH UNDERGOES SEQUENTIAL MALE TO FEMALE SEX REVERSAL WHICH OFFERS AN EXCELLENT SYSTEM TO STUDY THE ROLES OF SOMATIC CELLS AND GERM CELLS IN THE CONTEXT OF BOTH SEX DIFFERENTIATION AND SEX REVERSAL. THIS THESIS WORK WAS AIMED AT THE IDENTIFICATION AND CHARACTERIZATION OF MULTIPLE GERMLINE GENES FOR ANALYZING STAGE-SPECIFIC EXPRESSION DURING GAMETOGENESIS IN THE ASIAN SEABASS. GENES BOULE, DAZL AND VASA, WHICH ENCODE HIGHLY CONSERVED RNA BINDING PROTEINS, WERE IDENTIFIED BASED ON CLONING AND SEQUENCE ANALYSES. CHROMOGENIC IN SITU HYBRIDIZATION REVEALED THEIR GERM CELL-SPECIFIC EXPRESSION IN BOTH SEXES. TWO-COLOR FLUORESCENCE IN SITU HYBRIDIZATION (FISH) REVEALED DIFFERENTIAL TEMPOROSPATIAL PATTERNS OF VASA, DAZL AND BOULE RNA EXPRESSION TOGETHER WITH NUCLEAR STAINING, MORPHOLOGY AND TISSUE ARCHITECTURE AND ALLOWED FOR STAGING DEVELOPING GERM CELLS. FURTHERMORE, A THREE-COLOR FISH PROCEDURE WAS DEVELOPED THAT ALLOWS FOR SIMU
dc.language.isoen
dc.subjectAsian seabass, Germline, boule, dazl, vasa, Balbiani body
dc.typeThesis
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.supervisorHONG YUNHAN
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Ph.D Theses (Open)

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
1DwarakanathMA.pdf1.07 MBAdobe PDF

OPEN

NoneView/Download
2_1DwarakanathMA.pdf16.97 MBAdobe PDF

OPEN

NoneView/Download
2_2DwarakanathMA.pdf16.23 MBAdobe PDF

OPEN

NoneView/Download
2_3DwarakanathMA.pdf19.6 MBAdobe PDF

OPEN

NoneView/Download
2_4DwarakanathMA.pdf25.59 MBAdobe PDF

OPEN

NoneView/Download
3DwarakanathMA.pdf2.08 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

117
checked on May 22, 2020

Download(s)

115
checked on May 22, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.