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Title: Imaging Experience-Dependent Plasticity in the Mouse Barrel Cortex
Keywords: Optogenetics, Voltage-sensitive dye imaging, barrel cortex, somatosensory, critical period, circuits
Issue Date: 25-Sep-2014
Citation: LO SHUN QIANG (2014-09-25). Imaging Experience-Dependent Plasticity in the Mouse Barrel Cortex. ScholarBank@NUS Repository.
Abstract: Experience affects the development and function of somatosensory cortex circuits during specific time windows known as the critical period. However, the underlying circuit mechanisms are poorly understood. We used voltage-sensitive dye imaging and optogenetic circuit mapping to characterize experience-sensitive circuit changes occurring in the somatosensory cortex of whisker-deprived P30 mice. We found that deprivation weakened inhibitory circuits and reduced columnar specificity of excitatory circuits. To directly examine effects on parvalbumin (PV) expressing interneurons, we used a PV-Cre driver line, mated to another line with floxed-stop cassette controlling expression of the light-sensitive cation channel, Channelrhodopsin-2. Sensory deprivation decreased the range and amplitude of inhibitory postsynaptic input onto layer 2/3 pyramidal neurons. This effect on inhibitory circuits is age-sensitive, with the critical period closing around postnatal day 10. Our results provide a direct and quantitative measurement of the strength and spatial organization of this inhibitory circuit and its response to experience-dependent plasticity.
Appears in Collections:Ph.D Theses (Open)

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