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Title: Generation of Porcine Induced Pluripotent Stem Cells and their Differentiation into Cardiac Lineages
Keywords: Induced, pluripotent, stem, cells, porcine, differentiation
Issue Date: 24-Sep-2014
Citation: TAN WAN CHIU, GRACE (2014-09-24). Generation of Porcine Induced Pluripotent Stem Cells and their Differentiation into Cardiac Lineages. ScholarBank@NUS Repository.
Abstract: Heart disease is a major cause of death worldwide. As a result of myocardial infarction, functional cardiomyocytes are lost and this results in a heart that does not contract properly. The pig model is a good model to study cardiac functions in humans, as the size of the heart in the pig is similar to that of the human. In this project, we aim to convert porcine fibroblast cells into porcine cardiomyocytes, in the hope of creating new functional cardiomyocytes to replace the ones that are no longer functional after myocardial infarction. As no porcine embryonic stem cells have been isolated, induced pluripotent stem cells for the pig must be used as a source of pluripotent cells. Hence, fibroblasts from the pig is taken, and induced into pluripotent cells. These cells are in turn differentiated into cardiomyocytes, where more downstream work can be done to characterize them and also to use these cells as form of therapy for the failing heart. In this project, we isolated fibroblasts from the pig?s thigh region, and used lentiviruses to induce the cells to pluripotent cells. These pluripotent cells are studied and characterized. The pluripotent stem cells exhibited morphology and genomic markers of pluripotency. These cells were also finally differentiated into cardiomyocytes. Even though the differentiated cells did not beat, there were cardiac genes found to be expressed in the form of mRNA. This shows that the differentiation protocol is successful to a certain extent. More work can be done to streamline to protocol to achieve greater efficiency in the production of porcine cardiomyocytes
Appears in Collections:Master's Theses (Open)

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