Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/119210
Title: ADIPOGENIC INDUCTION UNDER MACROMOLECULAR CROWDING WITH FICOLL UNLOCKS THE INTRINSIC POTENTIAL IN ADULT HUMAN BONE MARROW MESENCHYMAL STEM CELLS TOWARDS A BROWN ADIPOCYTE PHENOTYPE
Authors: LEE HUI CHING MICHELLE
Keywords: adult human bone marrow, mesenchymal stem cells, cell differentiation, brown adipocytes, cell microenvironment, macromolecular crowding
Issue Date: 31-Oct-2014
Citation: LEE HUI CHING MICHELLE (2014-10-31). ADIPOGENIC INDUCTION UNDER MACROMOLECULAR CROWDING WITH FICOLL UNLOCKS THE INTRINSIC POTENTIAL IN ADULT HUMAN BONE MARROW MESENCHYMAL STEM CELLS TOWARDS A BROWN ADIPOCYTE PHENOTYPE. ScholarBank@NUS Repository.
Abstract: Brown adipose tissue holds therapeutic promise for diabetes and metabolic syndrome. Key to realizing this potential is the ability to generate functional human brown adipocytes from a readily available and sustainable cell source. Here, I demonstrate the generation of a functional brown phenotype from adult human bone marrow-derived mesenchymal stem cells (bmMSCs) under macromolecular crowding (MMC), a biophysical principle applied to cell culture in order to mimic a more crowded in vivo environment by sterically excluding volume and speeding up the rate of extracellular matrix formation. Adipogenic differentiation of bmMSCs yielded substantial UCP1 expression under MMC during the induction phase. The bmMSC-derived adipocytes generated under MMC were functionally capable of activating the thermogenic programme upon stimulation. MMC increased the deposition of collagen IV and heparan sulphate proteoglycans, indicative of a richer pro-adipogenic matrix, which would further drive adipogenesis through cell-matrix reciprocity. Additionally, bmMSC-derived white adipocytes could be `browned¿ under MMC.
URI: http://scholarbank.nus.edu.sg/handle/10635/119210
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