Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/118657
Title: TEMPORAL ASSOCIATION OF TENSIN1 AND P130CAS AT FOCAL ADHESIONS LINKS ACTOMYOSIN CONTRACTION TO CELL MIGRATION
Authors: TAN SONG HUI
Keywords: Tensin1, p130Cas, focal adhesions, actomyosin contraction, cell migration
Issue Date: 14-Aug-2014
Citation: TAN SONG HUI (2014-08-14). TEMPORAL ASSOCIATION OF TENSIN1 AND P130CAS AT FOCAL ADHESIONS LINKS ACTOMYOSIN CONTRACTION TO CELL MIGRATION. ScholarBank@NUS Repository.
Abstract: CELL MIGRATION IS A DYNAMIC PROCESS THAT PLAYS PIVOTAL ROLES IN BOTH PHYSIOLOGICAL AND PATHOLOGICAL PROCESSES. WE HAVE REPORTED THAT P130CAS (CRK-ASSOCIATED SUBSTRATE, HEREAFTER CAS) SUPPORTS CELL MIGRATION THROUGH THE BINDING TO SRC AS WELL AS PHOSPHORYLATION-DEPENDENT ASSOCIATION WITH ACTIN RETROGRADE FLOW AT FOCAL ADHESIONS. HOWEVER, IT REMAINS ELUSIVE HOW PHOSPHORYLATED CAS INTERACTS WITH ACTIN CYTOSKELETONS. WE OBSERVE THAT THE ACTIN-BINDING PROTEIN, TENSIN1, CO-LOCALIZES WITH CAS, BUT NOT ITS PHOSPHORYLATION-DEFECTIVE MUTANT AT THE CELL LAMELLIPODIA. WHILE A TRUNCATION MUTANT OF TENSIN1 THAT LACKS THE PHOSPHOTYROSINE-BINDING REGION, SH2PTB DOMAIN, DOES NOT CO-LOCALIZE WITH CAS, RECOMBINANT TENSIN1-SH2PTB PROTEIN BINDS TO CAS IN VITRO IS CAS PHOSPHORYLATION-DEPENDENT. THE EXOGENOUS EXPRESSION OF TENSIN1-SH2PTB, WHICH LACKS THE ACTIN-INTERACTING MOTIFS, INTERFERES WITH THE CAS-DRIVEN CELL MIGRATION, IMPEDES THE INWARD FLUX AND THE DISPLACEMENT OF CAS MOLECULES FROM FOCAL ADHESIONS.
URI: http://scholarbank.nus.edu.sg/handle/10635/118657
Appears in Collections:Master's Theses (Open)

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