Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/118652
Title: THE ROLE OF WHITE SPOT SYNDROME VIRAL PROTEIN VP9 IN HOST CHROMATIN ALTERATION
Authors: XI TAN
Keywords: viral protein, VP9, chromatin alteration, shrimp white spot syndrome
Issue Date: 15-Aug-2014
Citation: XI TAN (2014-08-15). THE ROLE OF WHITE SPOT SYNDROME VIRAL PROTEIN VP9 IN HOST CHROMATIN ALTERATION. ScholarBank@NUS Repository.
Abstract: White spot syndrome virus (WSSV) is a large double-stranded DNA virus that has caused serious diseases in crustaceans worldwide. The virus-host interaction is quite elusive due to the lack of WSSV susceptible cell lines. VP9 is one highly expressed nonstructural WSSV viral protein that might be involved in rerouting host cellular pathways to the benefit of the viral life cycle while its exact functional role has not been fully revealed. Previous studies have determined its 3D structure and shown its interaction with histone proteins. In this study, VP9 dynamics was quantitatively measured in the cytosol and nucleus of VP9-transfected cells. By single molecule force microscopy, we found that VP9 specifically impeded histone-mediated DNA folding by reducing histone?s binding affinity to DNA. Through salt fractionations and FRAP analysis, we detected the high proportion of soluble histone proteins, the formation of H3-VP9 complex and chromatin structure alternation in VP9-transfected cells. Microarray-based gene profiling further revealed that VP9 affects the global gene expression in Hela cells. These findings suggest that VP9 may epigenetically regulate host cell gene expression to facilitate WSSV infection and replication.
URI: http://scholarbank.nus.edu.sg/handle/10635/118652
Appears in Collections:Ph.D Theses (Open)

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