Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/118472
Title: FUNCTIONAL SIGNIFICANCE AND EXPRESSION ANALYSIS OF N-ACETYL GALACTOSAMINYL TRANSFERASE-1 IN BREAST CANCER
Authors: SIM WEY CHENG
Keywords: N-acetylgalactosaminyltransferase-1, CSGalNAcT-1, breast cancer, breast invasive ductal carcinoma, CXCL14, chondroitin sulfate
Issue Date: 23-Aug-2013
Citation: SIM WEY CHENG (2013-08-23). FUNCTIONAL SIGNIFICANCE AND EXPRESSION ANALYSIS OF N-ACETYL GALACTOSAMINYL TRANSFERASE-1 IN BREAST CANCER. ScholarBank@NUS Repository.
Abstract: BREAST CANCER IS THE TOP LEADING CAUSE OF CANCER AND MORTALITY IN WOMEN WORLDWIDE AND IN SINGAPORE. IN CURRENT STUDY, CSGALNACT-1 ENZYME, WHICH INITIATES THE BIOSYNTHESIS OF CHONDROITIN SULFATE, WAS FOUND UP-REGULATED IN NORMAL BREAST CELL LINE, MCF-12A; BUT DOWN-REGULATED IN BREAST CANCER CELL LINES, I.E. MCF7, ZR75-1 AND MDA-MB-231. HENCE, THE FUNCTIONAL ROLE OF CSGALNACT-1 WAS EXAMINED BY SILENCING AND OVER-EXPRESSING THIS GENE IN THE RESPECTIVE CELL LINES. RESULTS SHOWED THAT CSGALNACT-1 WAS INVOLVED IN REGULATING CELL MIGRATION, INVASION, SURVIVAL AND ADHESION. BESIDES, MICROARRAY ANALYSIS SHOWED THAT SILENCING CSGALNACT-1 RESULTED IN CHEMOKINE CXCL14 UP-REGULATION. IT WAS HYPOTHESIZED THAT CXCL14 MEDIATED THE PHENOTYPIC CHANGES IN MCF-12A DOWNSTREAM OF CSGALNACT-1 AND THIS WAS PROVEN THROUGH DOUBLE KNOCKDOWN EXPERIMENTS. IMMUNOHISTOCHEMISTRY WAS PERFORMED TO DETERMINE THE LOCALIZATION OF CSGALNACT-1 IN BREAST INVASIVE DUCTAL CARCINOMA CLINICAL SAMPLES. THE EXPRESSION OF CSGALNACT
URI: http://scholarbank.nus.edu.sg/handle/10635/118472
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