Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/118451
Title: SILK-BASED TISSUE ENGINEERED INTERVERTEBRAL DISC USING ADIPOSE-DERIVED STEM CELLS
Authors: NEO PUAY YONG
Keywords: tissue engineering, intervertebral disc, adipose-derived stem cells, silk
Issue Date: 18-Aug-2014
Citation: NEO PUAY YONG (2014-08-18). SILK-BASED TISSUE ENGINEERED INTERVERTEBRAL DISC USING ADIPOSE-DERIVED STEM CELLS. ScholarBank@NUS Repository.
Abstract: Tissue engineering a functional intervertebral disc (IVD) replacement offers a promising alternative treatment modality. The nucleus pulposus (NP) and annulus fibrosus (AF) work intimately to enable the IVD to function. This dissertation hence aims to develop a de novo disc consisting of both components for IVD replacement application. Adipose-tissue derived stem cells are used with silk-based biomaterials to recapitulate the NP and AF. The NP consists of silk/PVA cryogels, while the AF consists of electrospun-knitted silk laminates. The silk/PVA cryogels are mechanically robust and swellable, creating circumferential tension and radial compression forces on the surrounding AF. A bioreactor that can conduct load monitoring and compression is developed. Results of the de novo disc show the development of the AF?s ECM into a circumferential multi-ply like manner under compression, while its mechanical strength is comparable to reported values for native discs, attesting to its potential as a disc replacement option.
URI: http://scholarbank.nus.edu.sg/handle/10635/118451
Appears in Collections:Ph.D Theses (Open)

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