Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/118262
Title: CHARACTERIZATION OF NODAL ACTIN NETWORK: FUNCTIONS OF MYOSIN II, FILAMIN A AND FORMIN DAAM1
Authors: LUO WEIWEI
Keywords: Actin nodes; self-organized network; cytoskeleton cohesiveness; Myosin II; Filamin A; DAAM1; discrete stochastic computational model
Issue Date: 21-Aug-2014
Citation: LUO WEIWEI (2014-08-21). CHARACTERIZATION OF NODAL ACTIN NETWORK: FUNCTIONS OF MYOSIN II, FILAMIN A AND FORMIN DAAM1. ScholarBank@NUS Repository.
Abstract: Actin filaments, with the aid of multiple accessory proteins, self-assemble into a variety of network patterns. In the studies done by this thesis, the organization and dynamics of actin networks in adherent cells were investigated using pharmaceutical perturbation, live cell imaging, super-resolution imaging together with molecular and biochemistry analysis. A model of nodal organization of actin network was proposed. This cytoplasmic actin nodal network is formed by actin asters cross-linked and moved by non-muscle myosin II (NMII). The crosslinker filamin A (FlnA) and the formin DAAM1 were found to co-localize with the actin asters. Based on the experimental results, a computational model was developed. The model showed the dynamic formin-filamin-actin asters that can self-organize into a contractile actomyosin network. This cytoplasmic actin network maintains the coherence and the integrity of the cell, which is the basis for cell survival, migration and many physiological functions.
URI: http://scholarbank.nus.edu.sg/handle/10635/118262
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Movie 2.1 mpeg4 19fps.mov3.6 MBVideo Quicktime

OPEN

None Preview online
View/Download
Movie 3.1 Cell spreading 1_4 7fps.avi3.15 MBAVI

OPEN

None Preview online
View/Download
Movie 3.2 mpeg4 39fps.mov8.64 MBVideo Quicktime

OPEN

None Preview online
View/Download
Movie 3.3 MPEG4 19fps.mov3.04 MBVideo Quicktime

OPEN

None Preview online
View/Download
Movie 3.4 MPEG4 20fps.mov2.17 MBVideo Quicktime

OPEN

None Preview online
View/Download
Movie 4.1 19fps MPEG4 medium.mov5.65 MBVideo Quicktime

OPEN

None Preview online
View/Download
Movie 4.2 mpeg4 19fps.mov3.24 MBVideo Quicktime

OPEN

None Preview online
View/Download
Movie 5.1 DAAM1-233 vesicle movements 100x 1_2.avi2.64 MBAVI

OPEN

None Preview online
View/Download
Movie 5.2 LatA vs SMIFH2 100x.mov4.92 MBVideo Quicktime

OPEN

None Preview online
View/Download
Movie 5.3 Nodes in ctrl siRNA and Rap.avi16.13 MBAVI

OPEN

None Preview online
View/Download
Movie 5.4 LatAWashOffRapEffect_500x .avi2.91 MBAVI

OPEN

None Preview online
View/Download
Movie 5.5 Photoactivation 0.5x real time.avi6.91 MBAVI

OPEN

None Preview online
View/Download
Movie 6.1 quadCollapse.mov8.14 MBVideo Quicktime

OPEN

None Preview online
View/Download
Luo WW.pdf7.77 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

201
checked on Oct 23, 2020

Download(s)

203
checked on Oct 23, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.