Please use this identifier to cite or link to this item: https://doi.org/10.1016/S1089-8603(02)00103-9
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dc.titleDifferential expression and localization of nitric oxide synthases in cirrhotic livers of bile duct-ligated rats
dc.contributor.authorWei, C.-L.
dc.contributor.authorKhoo, H.-E.
dc.contributor.authorLee, K.-H.
dc.contributor.authorHon, W.-M.
dc.date.accessioned2014-12-17T08:55:05Z
dc.date.available2014-12-17T08:55:05Z
dc.date.issued2002
dc.identifier.citationWei, C.-L., Khoo, H.-E., Lee, K.-H., Hon, W.-M. (2002). Differential expression and localization of nitric oxide synthases in cirrhotic livers of bile duct-ligated rats. Nitric Oxide - Biology and Chemistry 7 (2) : 91-102. ScholarBank@NUS Repository. https://doi.org/10.1016/S1089-8603(02)00103-9
dc.identifier.issn10898603
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/117621
dc.description.abstractIncreased vascular nitric oxide (NO) production has been implicated in the pathogenesis of the hyperdynamic circulation in liver cirrhosis. This study investigated the expression of three isoforms of NO synthase (NOS) in rat cirrhotic livers. Cirrhosis was induced by chronic bile duct ligation (BDL). NOS enzyme activity was assessed by L-citrulline generation. Competitive RT-PCR was performed to detect the mRNA levels of NOS. In situ hybridization was done to localize NOS mRNA. Protein expression of NOS was evaluated by Western blotting and immunohistochemistry. The L-citrulline assay showed that constitutive NOS (cNOS) enzymatic activity was decreased, while inducible NOS (iNOS) activity was increased in BDL livers. Both endothelial NOS (eNOS) and neuronal NOS (nNOS) mRNA were detected in BDL and sham rats, but with enhanced expression in BDL rats. eNOS protein was redistributed with less expression in sinusoidal endothelial cells, but the total levels in liver were not changed, nNOS was induced in hepatocytes of BDL rats, in contrast to only a weak signal observed around some blood vessels in sham livers. Intense mRNA and protein expression of iNOS was induced in livers of BDL rats and was localized in hepatocytes, with no or a negligible amount in control livers. In conclusion, iNOS was induced in cirrhotic liver with its activity increased. In contrast, cNOS activity was impaired, regardless of unchanged eNOS protein levels and enhanced nNOS expression. These results suggest that all three types of NOS have a role in cirrhosis, but their expression and regulation are different. © 2002 Elsevier Science (USA). All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S1089-8603(02)00103-9
dc.sourceScopus
dc.subjectLiver cirrhosis
dc.subjectNitric oxide
dc.subjectNitric oxide synthase
dc.subjectProtein expression
dc.subjectQuantitative RT-PCR
dc.subjectRegulation
dc.typeArticle
dc.contributor.departmentMEDICINE
dc.contributor.departmentPAEDIATRICS
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/S1089-8603(02)00103-9
dc.description.sourcetitleNitric Oxide - Biology and Chemistry
dc.description.volume7
dc.description.issue2
dc.description.page91-102
dc.description.codenNIOXF
dc.identifier.isiut000178430000003
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