Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0042-6822(02)00136-8
DC FieldValue
dc.titleCD81 engineered with endocytotic signals mediates HCV cell entry: Implications for receptor usage by HCV in vivo
dc.contributor.authorTan, Y.-J.
dc.contributor.authorLim, S.-P.
dc.contributor.authorGoh, P.-Y.
dc.contributor.authorLim, S.G.
dc.contributor.authorTan, Y.H.
dc.contributor.authorHong, W.
dc.contributor.authorNg, P.
dc.date.accessioned2014-12-17T08:54:38Z
dc.date.available2014-12-17T08:54:38Z
dc.date.issued2003
dc.identifier.citationTan, Y.-J., Lim, S.-P., Goh, P.-Y., Lim, S.G., Tan, Y.H., Hong, W., Ng, P. (2003). CD81 engineered with endocytotic signals mediates HCV cell entry: Implications for receptor usage by HCV in vivo. Virology 308 (2) : 250-269. ScholarBank@NUS Repository. https://doi.org/10.1016/S0042-6822(02)00136-8
dc.identifier.issn00426822
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/117580
dc.description.abstractAlthough CD81 has been shown to bind HCV E2 protein, its role as a receptor for HCV remains controversial. In this study, we constructed two CD81 chimeras by linking the cytoplasmic domains of recycling surface receptors, low-density lipoprotein receptor (LDLR), and transferrin receptor (TfR), respectively, to CD81 and compared their internalization properties to wild-type CD81. Binding experiments with anti-hCD81 antibody showed that cell-surface CD81 chimeric receptors were internalized much more efficiently than wild-type CD81. In addition, CD81 chimeras, but not wild-type CD81, could internalize recombinant E2 protein and E2-enveloped viral particles from the serum of HCV-infected patients into Huh7 liver cells. The latter resulted in persistent positive-strand viral RNA and accumulation of replication intermediates, negative-strand viral RNA, in the infected cells, suggesting that the internalized viruses have undergone replication. Therefore, it appeared that CD81, possibly in association with a liver-specific endocytotic protein(s), represents one of the pathways by which HCV can infect hepatocytes. © 2003 Elsevier Science (USA). All rights reserved.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0042-6822(02)00136-8
dc.sourceScopus
dc.subjectCD81
dc.subjectChimeric receptors
dc.subjectEndocytosis
dc.subjectHepatitis C virus (HCV)
dc.subjectInternalization
dc.subjectLow-density lipoprotein receptor
dc.subjectTransferrin receptor
dc.subjectVirus replication
dc.typeArticle
dc.contributor.departmentMEDICINE
dc.contributor.departmentBIOCHEMISTRY
dc.description.doi10.1016/S0042-6822(02)00136-8
dc.description.sourcetitleVirology
dc.description.volume308
dc.description.issue2
dc.description.page250-269
dc.description.codenVIRLA
dc.identifier.isiut000182467400006
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

22
checked on Dec 2, 2021

WEB OF SCIENCETM
Citations

18
checked on Dec 2, 2021

Page view(s)

101
checked on Dec 2, 2021

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.