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Title: Microfabricated Environments to Study Collective Cell Behaviors
Authors: Vedula, S.R.K. 
Ravasio, A.
Anon, E.
Chen, T.
Peyret, G.
Ashraf, M.
Ladoux, B.
Keywords: Collective cell behavior
Epithelial gap closure
Geometrical constraints
Microcontact printing
Microfabricated substrates
Issue Date: 2014
Citation: Vedula, S.R.K., Ravasio, A., Anon, E., Chen, T., Peyret, G., Ashraf, M., Ladoux, B. (2014). Microfabricated Environments to Study Collective Cell Behaviors. Methods in Cell Biology 120 : 235-252. ScholarBank@NUS Repository.
Abstract: Coordinated cell movements in epithelial layers are essential for proper tissue morphogenesis and homeostasis. Microfabrication techniques have proven to be very useful for studies of collective cell migration in vitro. In this chapter, we briefly review the use of microfabricated substrates in providing new insights into collective cell behaviors. We first describe the development of micropatterned substrates to study the influence of geometrical constraints on cell migration and coordinated movements. Then, we present an alternative method based on microfabricated pillar substrates to create well-defined gaps within cell sheets and study gap closure. We also provide a discussion that presents possible pitfalls and sheds light onto the important parameters that allow the study of long-term cell culture on substrates of well-defined geometries. © 2014 Elsevier Inc.
Source Title: Methods in Cell Biology
ISSN: 0091679X
DOI: 10.1016/B978-0-12-417136-7.00016-1
Appears in Collections:Staff Publications

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