Please use this identifier to cite or link to this item: https://doi.org/10.1073/pnas.0904741106
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dc.titleMacromolecular crowding induced elongation and compaction of single DNA molecules confined in a nanochannel
dc.contributor.authorZhang, C.
dc.contributor.authorShao, P.G.
dc.contributor.authorVan Kan, J.A.
dc.contributor.authorVan Der Maarel, J.R.C.
dc.date.accessioned2014-12-12T08:01:07Z
dc.date.available2014-12-12T08:01:07Z
dc.date.issued2009-09-29
dc.identifier.citationZhang, C., Shao, P.G., Van Kan, J.A., Van Der Maarel, J.R.C. (2009-09-29). Macromolecular crowding induced elongation and compaction of single DNA molecules confined in a nanochannel. Proceedings of the National Academy of Sciences of the United States of America 106 (39) : 16651-16656. ScholarBank@NUS Repository. https://doi.org/10.1073/pnas.0904741106
dc.identifier.issn00278424
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/117067
dc.description.abstractThe effect of dextran nanoparticles on the conformation and compaction of single DNA molecules confined in a nanochannel was investigated with fluorescence microscopy. It was observed that the DNA molecules elongate and eventually condense into a compact form with increasing volume fraction of the crowding agent. Under crowded conditions, the channel diameter is effectively reduced, which is interpreted in terms of depletion in DNA segment density in the interfacial region next to the channel wall. Confinement in a nanochannel also facilitates compaction with a neutral crowding agent at low ionic strength. The threshold volume fraction for condensation is proportional to the size of the nanoparticle, due to depletion induced attraction between DNA segments. We found that the effect of crowding is not only related to the colligative properties of the agent and that confinement is also important. It is the interplay between anisotropic confinement and osmotic pressure which gives the elongated conformation and the possibility for condensation at low ionic strength.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1073/pnas.0904741106
dc.sourceScopus
dc.subjectDepletion
dc.subjectDextran
dc.subjectFluorescence
dc.subjectNanofluidics
dc.subjectNanoparticles
dc.typeArticle
dc.contributor.departmentNUS NANOSCIENCE & NANOTECH INITIATIVE
dc.contributor.departmentPHYSICS
dc.description.doi10.1073/pnas.0904741106
dc.description.sourcetitleProceedings of the National Academy of Sciences of the United States of America
dc.description.volume106
dc.description.issue39
dc.description.page16651-16656
dc.description.codenPNASA
dc.identifier.isiut000270305800022
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