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|Title:||Enhanced detection of early hepatocellular carcinoma by serum SELDI-TOF proteomic signature combined with alpha-fetoprotein marker||Authors:||Chen, L.
|Issue Date:||Sep-2010||Citation:||Chen, L., Ho, D.W.Y., Lee, N.P.Y., Sun, S., Lam, B., Wong, K.-F., Yi, X., Lau, G.K., Ng, E.W.Y., Poon, T.C.W., Lai, P.B.S., Cai, Z., Peng, J., Leng, X., Poon, R.T.P., Luk, J.M. (2010-09). Enhanced detection of early hepatocellular carcinoma by serum SELDI-TOF proteomic signature combined with alpha-fetoprotein marker. Annals of Surgical Oncology 17 (9) : 2518-2525. ScholarBank@NUS Repository. https://doi.org/10.1245/s10434-010-1038-8||Abstract:||Background: Biomarkers for accurate diagnosis of early hepatocellular carcinoma (HCC) are limited in number and clinical validation. We applied SELDI-TOF-MS ProteinChip technology to identify serum profile for distinguishing HCC and liver cirrhosis (LC) and to compare the accuracy of SELDI-TOF-MS profile and alpha-fetoprotein (AFP) level in HCC diagnosis. Patients and Methods: Serum samples were obtained from 120 HCC and 120 LC patients for biomarker discovery and validation studies. ProteinChip technology was employed for generating SELDI-TOF proteomic features and analyzing serum proteins/peptides. Results: A diagnostic model was established by CART algorithm, which is based on 5 proteomic peaks with m/z values at 3324, 3994, 4665, 4795, and 5152. In the training set, the CART algorithm could differentiate HCC from LC subjects with a sensitivity and specificity of 98% and 95%, respectively. The results were assessed in blind validation using separate cohorts of 60 HCC and 60 LC patients, with an accuracy of 83% for HCC and 92% for LC patients. The diagnostic odd ratio (DOR) indicated that SELDI-TOF proteomic signature could achieve better diagnostic performance than serum AFP level at a cutoff of 20 ng/mL (AFP20) (92.72 vs 9.11), particularly superior for early-stage HCC (87% vs 54%). Importantly, a combined use of both tests could enhance the detection of HCC (sensitivity, 95%; specificity, 98%; DOR, 931). Conclusion: Serum SELDI-TOF proteomic signature, alone or in combination with AFP marker, promises to be a good tool for early diagnosis and/screening of HCC in at-risk population with liver cirrhosis. © 2010 Society of Surgical Oncology.||Source Title:||Annals of Surgical Oncology||URI:||http://scholarbank.nus.edu.sg/handle/10635/117005||ISSN:||10689265||DOI:||10.1245/s10434-010-1038-8|
|Appears in Collections:||Staff Publications|
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