Please use this identifier to cite or link to this item:
Title: Bacillus anthracis tubulin-related protein Ba-TubZ assembles force-generating polymers
Authors: Srinivasan, R. 
Mishra, M.
Leong, F.Y.
Chiam, K.-H.
Balasubramanian, M.
Keywords: Bacteria
Fission yeast
Plasmid segregation
Issue Date: Sep-2011
Citation: Srinivasan, R., Mishra, M., Leong, F.Y., Chiam, K.-H., Balasubramanian, M. (2011-09). Bacillus anthracis tubulin-related protein Ba-TubZ assembles force-generating polymers. Cytoskeleton 68 (9) : 501-511. ScholarBank@NUS Repository.
Abstract: Pathogenicity of Bacillus anthracis depends on the faithful inheritance of plasmid pXO1, in a process that requires the plasmid encoded tubulin-related protein Ba-TubZ. Here we show, using heterologous expression in Schizosaccharomyces pombe, that Ba-TubZ assembles into a dynamic polymer in the absence of other B. anthracis proteins and can generate force capable of deforming the fission yeast nuclear envelope. The polymer bundles contain 27 ± 15 protofilaments/μm assuming that each protofilament spans the entire length. Thinner appearing buckled and thicker appearing straight filaments of Ba-TubZ were both capable of inducing nuclear envelope deformation. Unlike the related protein Bt-TubZ from Bacillus thuringiensis, which undergoes treadmilling upon expression in fission yeast, Ba-TubZ polymers did not undergo detectable treadmilling. Instead, in fluorescence recovery after photobleaching experiments, it displayed a different turnover behavior characterized by moderate fluorescence recovery along the entire length of the polymer. Modeling Ba-TubZ bundles as Euler-Bernoulli beams that buckle under compressive loads when pushed against the nuclear envelope allowed us to estimate that Ba-TubZ generates forces in the order of 1-10 nN. We propose that polymerization based filament elongation and force generation might aid faithful segregation of the virulence plasmid. © 2011 Wiley-Liss, Inc.
Source Title: Cytoskeleton
ISSN: 19493584
DOI: 10.1002/cm.20526
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.


checked on Oct 27, 2021


checked on Oct 27, 2021

Page view(s)

checked on Oct 14, 2021

Google ScholarTM



Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.