Please use this identifier to cite or link to this item:
|Title:||Bacillus anthracis tubulin-related protein Ba-TubZ assembles force-generating polymers||Authors:||Srinivasan, R.
|Issue Date:||Sep-2011||Citation:||Srinivasan, R., Mishra, M., Leong, F.Y., Chiam, K.-H., Balasubramanian, M. (2011-09). Bacillus anthracis tubulin-related protein Ba-TubZ assembles force-generating polymers. Cytoskeleton 68 (9) : 501-511. ScholarBank@NUS Repository. https://doi.org/10.1002/cm.20526||Abstract:||Pathogenicity of Bacillus anthracis depends on the faithful inheritance of plasmid pXO1, in a process that requires the plasmid encoded tubulin-related protein Ba-TubZ. Here we show, using heterologous expression in Schizosaccharomyces pombe, that Ba-TubZ assembles into a dynamic polymer in the absence of other B. anthracis proteins and can generate force capable of deforming the fission yeast nuclear envelope. The polymer bundles contain 27 ± 15 protofilaments/μm assuming that each protofilament spans the entire length. Thinner appearing buckled and thicker appearing straight filaments of Ba-TubZ were both capable of inducing nuclear envelope deformation. Unlike the related protein Bt-TubZ from Bacillus thuringiensis, which undergoes treadmilling upon expression in fission yeast, Ba-TubZ polymers did not undergo detectable treadmilling. Instead, in fluorescence recovery after photobleaching experiments, it displayed a different turnover behavior characterized by moderate fluorescence recovery along the entire length of the polymer. Modeling Ba-TubZ bundles as Euler-Bernoulli beams that buckle under compressive loads when pushed against the nuclear envelope allowed us to estimate that Ba-TubZ generates forces in the order of 1-10 nN. We propose that polymerization based filament elongation and force generation might aid faithful segregation of the virulence plasmid. © 2011 Wiley-Liss, Inc.||Source Title:||Cytoskeleton||URI:||http://scholarbank.nus.edu.sg/handle/10635/116932||ISSN:||19493584||DOI:||10.1002/cm.20526|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Oct 22, 2020
WEB OF SCIENCETM
checked on Oct 14, 2020
checked on Oct 23, 2020
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.