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|Title:||Pleomorphomonas diazotrophica sp. nov., an endophytic N-fixing bacterium isolated from root tissue of Jatropha curcas L.||Authors:||Madhaiyan, M.
|Issue Date:||1-Jul-2013||Citation:||Madhaiyan, M., Jin, T.Y., Roy, J.J., Kim, S.-J., Weon, H.-Y., Kwon, S.-W., Ji, L. (2013-07-01). Pleomorphomonas diazotrophica sp. nov., an endophytic N-fixing bacterium isolated from root tissue of Jatropha curcas L.. International Journal of Systematic and Evolutionary Microbiology 63 (PART7) : 2477-2483. ScholarBank@NUS Repository. https://doi.org/10.1099/ijs.0.044461-0||Abstract:||A novel aerobic, non-motile, pleomorphic, Gram-negative and nitrogen-fixing bacterial strain, designated R5-392T, was isolated from surface-sterilized root tissue of Jatropha curcas. The organism grew optimally at 30 °C in media containing 1 % (w/v) NaCl and at pH 6.0-8.0. The predominant ubiquinone was Q-10 and the major fatty acids were C18: 1ω7c/C18: 1ω6c, C16: 0 and C19: 0 cyclo ω8c. The DNA G+C content was 63.2 mol%. Analysis of the 16S rRNA gene sequence suggested that strain R5-392T is affiliated with the order Rhizobiales within the class Alphaproteobacteria and is most closely related to Pleomorphomonas oryzae F-7T (98.8 % similarity) and Pleomorphomonas koreensis Y9T (98.3 % similarity). Analysis of partial nifH gene sequences also revealed a monophyletic lineage within the class Alphaproteobacteria, and strain R5-392T was most closely related to P. oryzae F-7T (98 %). Highest nitrogenase activity was detected in the presence of low-level organic nitrogen or in the presence of nitrogenase co-factors (Fe/Mo) in N-free media. Phenotypic and chemotaxonomic data suggest that strain R5-392T represents a novel species within the genus Pleomorphomonas, for which the name Pleomorphomonas diazotrophica sp. nov. is proposed. The type strain is R5-392T (= KACC 16233T = DSM 25022T). © 2013 IUMS.||Source Title:||International Journal of Systematic and Evolutionary Microbiology||URI:||http://scholarbank.nus.edu.sg/handle/10635/116529||ISSN:||14665026||DOI:||10.1099/ijs.0.044461-0|
|Appears in Collections:||Staff Publications|
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