Please use this identifier to cite or link to this item:
Title: P element-mediated germ-line transformation of Drosophila.
Authors: O'Connor, M. 
Chia, W.
Issue Date: 1993
Citation: O'Connor, M.,Chia, W. (1993). P element-mediated germ-line transformation of Drosophila.. Methods in molecular biology (Clifton, N.J.) 18 : 75-85. ScholarBank@NUS Repository.
Abstract: In contrast to many organisms, Drosophila embryos do not integrate injected DNA at an appreciable frequency. For this reason, the generation of germ-line transformants has relied on the utilization of transposable elements to effect the chromosomal integration of injected DNA (1,2). The success of this approach has depended largely on our understanding of the biology of P elements and the syncitial nature of the early Drosophila embryo. The first 13 embryonic divisions following fertilization are nuclear, resulting in the formation of a syncitium. Consequently, if microinjection into the posterior end of the embryo is carried out prior to cellularization, a proportion of the microinjected DNA will be present in the cytoplasm of the pole cells, the progenitor cells of the germ line.
Source Title: Methods in molecular biology (Clifton, N.J.)
ISSN: 19406029
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Oct 11, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.