Please use this identifier to cite or link to this item: https://doi.org/10.1007/978-1-60327-977-2_12
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dc.titleZebrafish spotted-microarray for genome-wide expression profiling experiments. Part I: array printing and hybridization.
dc.contributor.authorLam, S.H.
dc.contributor.authorMathavan, S.
dc.contributor.authorGong, Z.
dc.date.accessioned2014-12-12T07:35:34Z
dc.date.available2014-12-12T07:35:34Z
dc.date.issued2009
dc.identifier.citationLam, S.H.,Mathavan, S.,Gong, Z. (2009). Zebrafish spotted-microarray for genome-wide expression profiling experiments. Part I: array printing and hybridization.. Methods in molecular biology (Clifton, N.J.) 546 : 175-195. ScholarBank@NUS Repository. <a href="https://doi.org/10.1007/978-1-60327-977-2_12" target="_blank">https://doi.org/10.1007/978-1-60327-977-2_12</a>
dc.identifier.issn10643745
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/116034
dc.description.abstractThe availability of microarray technology for zebrafish research has enabled the expression of tens of thousands of genes to be studied simultaneously in one experiment. The experiment usually involves measuring and comparing the relative abundance of tens of thousands of mRNA species in experimental samples obtained from mutant versus wild-type embryos, disease versus normal tissues, embryos/fish of different developmental stages, physiologic states, or from multiple treatments and/or time-points. A microarray experiment comprised of several stages can be divided into two distinct parts (i.e., the "wet-lab" and the "dry-lab"). The success of a microarray experiment hinges on the "wet-lab" procedures, which include technology that allows for generation of arrays with very high-density DNA where tens of thousands of genes are represented in an area smaller than a standard glass microscope slide, and procedures that enable extraction of high-quality RNA, efficient fluorescent labeling of nucleic acids, as well as specific hybridization of fluorescent labeled-samples with arrayed probes. This chapter describes these "wet-lab" procedures. "Dry-lab" procedures are described in the next chapter.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1007/978-1-60327-977-2_12
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentGENOME INSTITUTE OF SINGAPORE
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1007/978-1-60327-977-2_12
dc.description.sourcetitleMethods in molecular biology (Clifton, N.J.)
dc.description.volume546
dc.description.page175-195
dc.identifier.isiutNOT_IN_WOS
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