Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0303-7207(99)00193-8
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dc.titleTemporal expression and T3 induction of thyroid hormone receptors α1 and β1 during early embryonic and larval development in zebrafish, Danio rerio
dc.contributor.authorLiu, Y.-W.
dc.contributor.authorLo, L.-J.
dc.contributor.authorChan, W.-K.
dc.date.accessioned2014-12-12T07:34:46Z
dc.date.available2014-12-12T07:34:46Z
dc.date.issued2000-01-25
dc.identifier.citationLiu, Y.-W., Lo, L.-J., Chan, W.-K. (2000-01-25). Temporal expression and T3 induction of thyroid hormone receptors α1 and β1 during early embryonic and larval development in zebrafish, Danio rerio. Molecular and Cellular Endocrinology 159 (1-2) : 187-195. ScholarBank@NUS Repository. https://doi.org/10.1016/S0303-7207(99)00193-8
dc.identifier.issn03037207
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/115970
dc.description.abstractThe effects of thyroid hormones on metabolism and development are mediated by thyroid hormone receptors (TRs). We report the cloning and characterization of a TRβ1 cDNA from zebrafish. Southern blot analysis revealed that there is a single genomic locus for the TRβ gene, while the TRα gene potentially has two loci. Multiple TRα and TRβ transcripts were detected in adult tissues. Using a semiquantitative RT-PCR assay, zygotic expression of TRα1 and TRβ1 were shown to occur before the midblastula transition stage. In transiently transfected HeLa cells, TRα1 displayed constitutive transactivation in the absence of ligands, which was slightly enhanced by triiodothyronine (T3). The transactivating activity of TRβ1 was strictly ligand-dependent and repressed in the absence of T3. Finally, the T3 induction of TRα1 and TRβ1 mRNAs was demonstrated in zebrafish embryos and larvae. The auto-induction of TRα1 and TRβ1 may serve a regulatory role during the embryonic and larval development of zebrafish. (C) 2000 Elsevier Science Ireland Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0303-7207(99)00193-8
dc.sourceScopus
dc.subjectEmbryonic development
dc.subjectLarvae
dc.subjectThyroid hormone receptor
dc.subjectZebrafish
dc.typeArticle
dc.contributor.departmentINSTITUTE OF MOLECULAR AGROBIOLOGY
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.description.doi10.1016/S0303-7207(99)00193-8
dc.description.sourcetitleMolecular and Cellular Endocrinology
dc.description.volume159
dc.description.issue1-2
dc.description.page187-195
dc.description.codenMCEND
dc.identifier.isiut000084969400019
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