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Title: Status epilepticus alters hippocampal PKAβ and PKAγ expression in mice
Authors: Liu, J.X.
Tang, Y.C.
Liu, Y.
Tang, F.R. 
Keywords: Hippocampus
Status epilepticus
Issue Date: Sep-2010
Citation: Liu, J.X., Tang, Y.C., Liu, Y., Tang, F.R. (2010-09). Status epilepticus alters hippocampal PKAβ and PKAγ expression in mice. Seizure 19 (7) : 414-420. ScholarBank@NUS Repository.
Abstract: Objectives: To investigate the localization and progressive changes of cyclic-AMP dependent protein kinase (cPKA) in the mouse hippocampus at acute stages during and after pilocarpine induced status epilepticus. Methods: Pilocarpine induced status epilepticus mice were sacrificed 30 min, 2 h or 1 day after the start of a ∼7 h lasting status as assessed by video-electroencephalography. Brains were processed for quantitative immunohistochemistry of hippocampal cPKAβ and cPKAγ, and immunohistochemical co-localization of cPKAβ and cPKAγ with calbindin (CB), calretinin (CR), and parvalbumin (PV). Results: Based on anatomical and morphological assessment, cPKAβ was primarily expressed by principal cells and cPKAγ by interneurons. In CA1, cPKAβ co-localized with 76% of CB, 41% of CR, and 95% of PV-immunopositive cells, while cPKAγ co-localized with 50% of CB, 29% of CR, and 80% of PV-immunopositive cells. Upon induction of status epilepticus, cPKAβ expression was transiently reduced in CA1, whereas cPKAγ expression was sustainably reduced. Conclusion: cPKA may play an important role in neuronal hyperexcitability, death and epileptogenesis during and after pilocarpine induced status epilepticus. © 2010 British Epilepsy Association. Published by Elsevier Ltd. All rights reserved.
Source Title: Seizure
ISSN: 10591311
DOI: 10.1016/j.seizure.2010.06.008
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