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https://doi.org/10.1016/j.seizure.2010.06.008
Title: | Status epilepticus alters hippocampal PKAβ and PKAγ expression in mice | Authors: | Liu, J.X. Tang, Y.C. Liu, Y. Tang, F.R. |
Keywords: | Hippocampus Mouse PKA Status epilepticus |
Issue Date: | Sep-2010 | Citation: | Liu, J.X., Tang, Y.C., Liu, Y., Tang, F.R. (2010-09). Status epilepticus alters hippocampal PKAβ and PKAγ expression in mice. Seizure 19 (7) : 414-420. ScholarBank@NUS Repository. https://doi.org/10.1016/j.seizure.2010.06.008 | Abstract: | Objectives: To investigate the localization and progressive changes of cyclic-AMP dependent protein kinase (cPKA) in the mouse hippocampus at acute stages during and after pilocarpine induced status epilepticus. Methods: Pilocarpine induced status epilepticus mice were sacrificed 30 min, 2 h or 1 day after the start of a ∼7 h lasting status as assessed by video-electroencephalography. Brains were processed for quantitative immunohistochemistry of hippocampal cPKAβ and cPKAγ, and immunohistochemical co-localization of cPKAβ and cPKAγ with calbindin (CB), calretinin (CR), and parvalbumin (PV). Results: Based on anatomical and morphological assessment, cPKAβ was primarily expressed by principal cells and cPKAγ by interneurons. In CA1, cPKAβ co-localized with 76% of CB, 41% of CR, and 95% of PV-immunopositive cells, while cPKAγ co-localized with 50% of CB, 29% of CR, and 80% of PV-immunopositive cells. Upon induction of status epilepticus, cPKAβ expression was transiently reduced in CA1, whereas cPKAγ expression was sustainably reduced. Conclusion: cPKA may play an important role in neuronal hyperexcitability, death and epileptogenesis during and after pilocarpine induced status epilepticus. © 2010 British Epilepsy Association. Published by Elsevier Ltd. All rights reserved. | Source Title: | Seizure | URI: | http://scholarbank.nus.edu.sg/handle/10635/115949 | ISSN: | 10591311 | DOI: | 10.1016/j.seizure.2010.06.008 |
Appears in Collections: | Staff Publications |
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