Please use this identifier to cite or link to this item: https://doi.org/10.1002/jms.3150
DC FieldValue
dc.titleQuantification of L-ergothioneine in human plasma and erythrocytes by liquid chromatography-tandem mass spectrometry
dc.contributor.authorWang, L.-Z.
dc.contributor.authorThuya, W.-L.
dc.contributor.authorToh, D.S.-L.
dc.contributor.authorLie, M.G.-L.
dc.contributor.authorLau, J.-Y.A.
dc.contributor.authorKong, L.-R.
dc.contributor.authorWan, S.-C.
dc.contributor.authorChua, K.-N.
dc.contributor.authorLee, E.J.-D.
dc.contributor.authorGoh, B.-C.
dc.date.accessioned2014-12-12T07:33:49Z
dc.date.available2014-12-12T07:33:49Z
dc.date.issued2013-03
dc.identifier.citationWang, L.-Z., Thuya, W.-L., Toh, D.S.-L., Lie, M.G.-L., Lau, J.-Y.A., Kong, L.-R., Wan, S.-C., Chua, K.-N., Lee, E.J.-D., Goh, B.-C. (2013-03). Quantification of L-ergothioneine in human plasma and erythrocytes by liquid chromatography-tandem mass spectrometry. Journal of Mass Spectrometry 48 (3) : 406-412. ScholarBank@NUS Repository. https://doi.org/10.1002/jms.3150
dc.identifier.issn10765174
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/115894
dc.description.abstractA sensitive analytical method has been developed and validated for the quantification of L-ergothioneine in human plasma and erythrocytes by liquid chromatography-tandem mass spectrometry. A commercially available isotope-labeled L-ergothioneine-d9 is used as the internal standard. A simple protein precipitation with acetonitrile is utilized for bio-sample preparation prior to analysis. Chromatographic separation of L-ergothioneine is conducted using gradient elution on Alltime C18 (150 mm × 2.1 mm, 5 Âμ). The run time is 6 min at a constant flow rate of 0.45 ml/min. The mass spectrometer is operated under a positive electrospray ionization condition with multiple reaction monitoring mode. The mass transitions of L-ergothioneine and L-ergothioneine-d9 are m/z 230 > 127 and m/z 239 > 127, respectively. Excellent linearity [coefficient of determination (r2) ≥ 0.9998] can be achieved for L-ergothioneine quantification at the ranges of 10 to 10 000 ng/ml, with the intra-day and inter-day precisions at 0.9-3.9% and 1.3-5.7%, respectively, and the accuracies for all quality control samples between 94.5 and 101.0%. This validated analytical method is suitable for pharmacokinetic monitoring of L-ergothioneine in human and erythrocytes. Based on the determination of bio-samples from five healthy subjects, the mean concentrations of L-ergothioneine in plasma and erythrocytes are 107.4 ± 20.5 ng/ml and 1285.0 ± 1363.0 ng/ml, respectively. Copyright © 2013 John Wiley & Sons, Ltd.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1002/jms.3150
dc.sourceScopus
dc.subjecterythrocyte
dc.subjecthuman
dc.subjectL-ergothioneine
dc.subjectLC-MS/MS
dc.subjectplasma
dc.typeArticle
dc.contributor.departmentCANCER SCIENCE INSTITUTE OF SINGAPORE
dc.description.doi10.1002/jms.3150
dc.description.sourcetitleJournal of Mass Spectrometry
dc.description.volume48
dc.description.issue3
dc.description.page406-412
dc.description.codenJMSPF
dc.identifier.isiut000316293400019
Appears in Collections:Staff Publications

Show simple item record
Files in This Item:
There are no files associated with this item.

SCOPUSTM   
Citations

9
checked on Sep 19, 2020

WEB OF SCIENCETM
Citations

9
checked on Sep 11, 2020

Page view(s)

112
checked on Sep 19, 2020

Google ScholarTM

Check

Altmetric


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.