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Title: Morphological and genetic diversity of symbiotic cyanobacteria from cycads
Authors: Thajuddin, N.
Muralitharan, G.
Sundaramoorthy, M.
Ramamoorthy, R.
Ramachandran, S. 
Akbarsha, M.A.
Gunasekaran, M.
Keywords: 16S rRNA gene sequence
Issue Date: Jun-2010
Citation: Thajuddin, N., Muralitharan, G., Sundaramoorthy, M., Ramamoorthy, R., Ramachandran, S., Akbarsha, M.A., Gunasekaran, M. (2010-06). Morphological and genetic diversity of symbiotic cyanobacteria from cycads. Journal of Basic Microbiology 50 (3) : 254-265. ScholarBank@NUS Repository.
Abstract: The morphological and genetic diversity of cyanobacteria associated with cycads was examined using PCR amplification techniques and 16S rRNA gene sequence analysis. Eighteen symbiotic cyanobacteria were isolated from different cycad species. One of the symbiotic isolates was a species of Calothrix, a genus not previously reported to form symbioses with Cycadaceae family, and the remainder were Nostoc spp. Axenic cyanobacterial strains were compared by DNA amplification using PCR with either short arbitrary primers or primers specific for the repetitive sequences. Based on fingerprint patterns and phenograms, it was revealed that cyanobacterial symbionts exhibit important genetic diversity among host plants, both within and between cycad populations. A phylogenetic analysis based on 16S rRNA gene sequence analysis revealed that most of the symbiotic cyanobacterial isolates fell into well-separated clades. © 2010 WILEY-VCH Verlag GmbH & Co.
Source Title: Journal of Basic Microbiology
ISSN: 0233111X
DOI: 10.1002/jobm.200900343
Appears in Collections:Staff Publications

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