Please use this identifier to cite or link to this item:
|Title:||Intracellular localization and intercellular heterogeneity of the human DNA repair protein O6-methylguanine-DNA methyltransferase||Authors:||Belanich, M.
Schold Jr., S.C.
|Issue Date:||1996||Citation:||Belanich, M., Randall, T., Pastor, M.A., Kibitel, J.T., Alas, L.G., Dolan, M.E., Schold Jr., S.C., Gander, M., Lejeune, F.J., Li, B.F.L., White, A.B., Wasserman, P., Citron, M.L., Yarosh, D.B. (1996). Intracellular localization and intercellular heterogeneity of the human DNA repair protein O6-methylguanine-DNA methyltransferase. Cancer Chemotherapy and Pharmacology 37 (6) : 547-555. ScholarBank@NUS Repository. https://doi.org/10.1007/s002800050427||Abstract:||O6-Methylguanine-DNA methyltransferase (MGMT) is a DNA repair protein that removes alkyl adducts from DNA and may be important in tumor resistance to alkylation chemotherapy. MGMT was visualized in human cells and tumor tissues with monoclonal antibodies against MGMT and immunofluorescence microscopy, and fluorescent signals were quantified by digital image analysis. MGMT was found both in the cytoplasm and the nucleus, and in either locale the protein reacts with alkylated DNA bases and becomes inactivated and lost from the cell. Cell lines in culture and xenografts showed a broad normal distribution of nuclear MGMT levels, but human brain tumors often showed a skewed distribution, with a significant fraction of cells with high levels of MGMT. O6-Benzylguanine, a suicide substrate inactivator for MGMT activity, reduced MGMT in human cells and in a mouse xenograft to levels undetectable by antibody assay 1 h post-treatment. In melanoma specimens taken from a patient 3 h post-treatment with temozolomide, MGMT levels were reduced by 70%. This quantitative immunofluorescence assay can be used to monitor MGMT and its depletion in human tumors to improve the use of alkylating agents in cancer chemotherapy.||Source Title:||Cancer Chemotherapy and Pharmacology||URI:||http://scholarbank.nus.edu.sg/handle/10635/115780||ISSN:||03445704||DOI:||10.1007/s002800050427|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Jan 31, 2023
WEB OF SCIENCETM
checked on Jan 31, 2023
checked on Feb 2, 2023
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.